Manganese superoxide dismutase exon 3-deleted isoforms and nucleic acid molecules encoding the isoforms

Abstract

A new isoform of manganese superoxide dismutase (MnSOD) and polynucleotides encoding it have been identified. This isoform, MnSOD E3(−), is a splice variant lacking exon 3 of the full length MnSOD. The polypeptide can be expressed using appropriate host cells. Modulation of either the expression of the polynucleotides of the activity of the polypeptide is also described. Furthermore, diagnostic and therapeutic methods have been developed as a consequence of the isolation of the polynucleotides and polypeptides.

1 - 30 . (canceled) 
     
     
         31 . A non-human transgenic animal that overexpresses exon 3-deleted MnSOD, or a variant thereof having the same activity. 
     
     
         32 . The non-human transgenic animal of claim  1 , wherein said transgenic animal comprises an exogenous nucleic acid encoding exon 3-deleted MnSOD, or a variant thereof having the same activity. 
     
     
         33 . The non-human transgenic animal of claim  2  wherein said exon 3-deleted MnSOD comprises the sequence of SEQ ID NO:2. 
     
     
         34 . The non-human transgenic animal of claim  2  wherein said nucleic acid comprises the sequence of SEQ ID NO:1. 
     
     
         35 . The non-human transgenic animal of claim  1  wherein said animal is a rodent. 
     
     
         36 . The non-human transgenic animal of claim  5  wherein the animal is a mouse. 
     
     
         37 . A non-human transgenic animal that is a knock-out for exon 3-deleted MnSOD. 
     
     
         38 . The non-human transgenic animal of claim  7  wherein the gene encoding exon 3-deleted MnSOD has been knocked-out. 
     
     
         39 . The non-human transgenic animal of claim  7  wherein said animal is a rodent. 
     
     
         40 . The non-human transgenic animal of claim  8  wherein said animal is a mouse.