Methods for using soy peptides to inhibit h3 acetylation, reduce expression of hmg-coa reductase and increase ldl receptor and sp1 expression in a mammal

Abstract

Controlled studies demonstrate that methods using soy related peptides inhibit H3 acetylation, reduce expression of HMG-CoA reductase and increase LDL receptor and Sp1 expression in mammals. The present disclosure is generally directed to using lunasin peptides and/or lunasin peptide derivatives to 1) inhibit H3 acetylation, 2) reduce expression of HMG-CoA reductase, 3) increase LDL receptor expression or 4) increase Sp1 expression in a mammal. In at least one exemplary embodiment of the present disclosure, an effective amount of lunasin peptides or lunasin peptide derivatives and one or more enzyme inhibitors is provided to a mammal to 1) inhibit H3 acetylation, 2) reduce expression of HMG-CoA reductase, 3) increase LDL receptor expression or 4) increase Sp1 expression in a mammal. Additionally, lunasin will protect against, prevent, or reduce: 1) the expression of Matrix metalloproteinase (MMP-1), 2) collagen breakdown, 3) photoaging and 4) the formation of skin wrinkles.

1 . A method for protecting against photoaging of skin in an individual, comprising: (a) providing: (i) an individual desiring to prevent photoaging of skin and, (ii) a composition comprising a compound selected from the group consisting of the peptide of SEQ ID NO 2 and a functionally equivalent variant, fragment or analogue of said peptide; and (b) administering said composition to said subject to protect against photoaging. 
     
     
         2 . The method of  claim 1 , wherein said compound is obtained from soybean, wheat or barley. 
     
     
         3 . The method of  claim 1 , wherein said compound is obtained by producing, extracting and purifying said compound using recombinant DNA techniques. 
     
     
         4 . The method of  claim 1 , wherein said compound is obtained by synthetic polypeptide production. 
     
     
         5 . The method of  claim 1 , wherein said individual is a human. 
     
     
         6 . The method of  claim 1  wherein administering comprises topical administration of the composition. 
     
     
         7 . The method of  claim 1 , wherein the composition is in the form of a semi-solid formulation, liquid, gel, suspension, or aerosol spray. 
     
     
         8 . The method of  claim 1 , wherein said composition further comprises chymotrypsin inhibitor. 
     
     
         9 . The method of  claim 1 , wherein said compound is administered to said individual at between 5 μg/ml and 50 μg/ml. 
     
     
         10 . A method for protecting against collagen breakdown in the skin of an individual, comprising: (a) providing: (i) an individual desiring to prevent collagen breakdown in the skin and, (ii) a composition comprising a compound selected from the group consisting of the peptide of SEQ ID NO 2 and a functionally equivalent variant, fragment or analogue of said peptide; and (b) administering said composition to said subject to protect against collagen breakdown. 
     
     
         11 . The method of  claim 10 , wherein said compound is obtained from soybean, wheat or barley. 
     
     
         12 . The method of  claim 10 , wherein said compound is obtained by producing, extracting and purifying said compound using recombinant DNA techniques. 
     
     
         13 . The method of  claim 10 , wherein said compound is obtained by synthetic polypeptide production. 
     
     
         14 . The method of  claim 10 , wherein said individual is a human. 
     
     
         15 . The method of  claim 10  wherein administering comprises topical administration of the composition. 
     
     
         16 . The method of  claim 10 , wherein the composition is in the form of a semi-solid formulation, liquid, gel, suspension, or aerosol spray. 
     
     
         17 . The method of  claim 10 , wherein said composition further comprises chymotrypsin inhibitor. 
     
     
         18 . The method of  claim 10 , wherein said compound is administered to said individual at between 5 μg/ml and 50 μg/ml. 
     
     
         19 . A method for protecting against wrinkling of the skin in an individual, comprising: (a) providing: (i) an individual desiring to prevent wrinkling of skin and, (ii) a composition comprising a compound selected from the group consisting of the peptide of SEQ ID NO 2 and a functionally equivalent variant, fragment or analogue of said peptide; and (b) administering said composition to said subject to protect against wrinkling of the skin. 
     
     
         20 . The method of  claim 19 , wherein said compound is obtained from soybean, wheat or barley. 
     
     
         21 . The method of  claim 19 , wherein said compound is obtained by producing, extracting and purifying said compound using recombinant DNA techniques. 
     
     
         22 . The method of  claim 19 , wherein said compound is obtained by synthetic polypeptide production. 
     
     
         23 . The method of  claim 19 , wherein said individual is a human. 
     
     
         24 . The method of  claim 19  wherein administering comprises topical administration of the composition. 
     
     
         25 . The method of  claim 19 , wherein the composition is in the form of a semi-solid formulation, liquid, gel, suspension, or aerosol spray. 
     
     
         26 . The method of  claim 19 , wherein said composition further comprises chymotrypsin inhibitor. 
     
     
         27 . The method of  claim 19 , wherein said compound is administered to said individual at between 5 μg/ml and 50 μg/ml.