Ablation based laser machining of biomolecule patterns on substrates

Abstract

A method for patterning a one or more biomolecules on a substrate that includes coating the substrate with a coating of the one or more biomolecules, applying a laser to the coating, and ablating a portion of the one or more biomolecules with the laser in a predetermined pattern.

1 - 15 . (canceled) 
   
   
       16 . A substrate coated with one or more biomolecules in a pattern comprising:
 a first portion on the substrate having a coating, the coating having the one or more biomolecules;   one or more ablated portions on the substrate, the one or more ablated portions having at least one dimension in the plane of the substrate from at least about 0.1 nanometer, at least about 1 nanometer, at least about 10 nanometers, at least about 100 nanometers, or at least about 250 nanometers to about 1 meter; and   one or more non-ablated portions on the substrate, the one or more non-ablated portions having at least one dimension in the plane of the substrate from about at least about 0.1 nanometer, at least about 1 nanometer, at least about 10 nanometers, at least about 100 nanometers, or at least about 250 nanometers to about 1 meter, wherein the one or more ablated portions have less than 100% of biological function or activity, or biochemical function or activity of the one or more biomolecules on the substrate.   
   
   
       17 . The substrate of  claim 16 , wherein the substrate coated with the one or more biomolecules is selected from the group consisting of a microscope coverslip, microscope slide, petri dish, tissue culture flask, biomedical implant, test tube, eppendorf tube, diagnostic assay, a biochip, a protein/nucleic acid biochip sensor, a cell-based sensor, a lab-on-a-chip assay, a lab-in-a-capillary assay, a cell adhesion assay, a cell 
     translocation/migration/invasion/chemotaxis assay, or a neuronal-guidance assay. 
   
   
       18 . The substrate of  claim 16 , wherein the substrate has an exterior side opposite an interior side, and wherein the interior side has the coating applied thereon. 
   
   
       19 . The substrate of  claim 16 , wherein the one or more biomolecules are in a dry environment, hydrated by a layer of liquid, in an aqueous solvent environment or in a non-aqueous solvent environment. 
   
   
       20 . The substrate of  claim 16 , wherein the one or more biomolecules are selected from the group consisting of proteins, peptides, nucleic acids, drugs, lipids, and any combination thereof. 
   
   
       21 . The substrate of  claim 16 , wherein the coating comprises attaching the one or more biomolecules on the substrate by a method selected from the group consisting of covalently attaching to the substrate, adsorbing to the substrate, attaching via a coupling molecule of varying length, electrostatically attaching to the substrate, hydrophobically attaching to the substrate, sterically attaching to the substrate, entropically attaching to the substrate, and any combination thereof. 
   
   
       22 . The substrate of  claim 16 , wherein the one or more of biomolecules are attached to a particle or colloid selected from the group consisting of quantum dots, superparamagnetic nanoparticles, dendrimers, glass or silica particles, liposomes, viruses or phage particles and analogous particles, and any combination thereof. 
   
   
       23 . The substrate of  claim 16 , wherein the substrate is selected from the group consisting of silicon, plastic, rubber, metal, ceramic material, or any material that is not destroyed or damaged by the laser, and any combination thereof, wherein the material that is not substantially destroyed or damaged comprises material that is not substantially destroyed or damaged when laser irradiation of an area of substrate  10  removes material to a depth of more than 1 nanometer, more than 3 nanometer, more than 5 nanometers, more than 10 nanometers, more than 25 nanometers, more than 50 nanometers, more than 100 nanometers, more than 500 nanometers, more than 1 micrometer, more than 5 micrometers, more than 10 micrometers, more than 50 micrometers, more than 100 micrometers from a point in an area irradiated by the laser. 
   
   
       24 . A patterned substrate coated with a one or more biomolecules comprising:
 a pattern obtainable by coating the substrate with the one or more biomolecules, applying a laser onto the one or more of molecules, and ablating a portion of the one or more biomolecules with the laser in a predetermined pattern, the predetermined pattern having one or more ablated portions and one or more non-ablated portions on the substrate, the one or more ablated portions having less than 100% of biological function or activity, or biochemical function or activity of the one or more biomolecules on the substrate, and the one or more ablated portions having one or more active biomolecules of the one or more biomolecules on the substrate.