Method of electrically detecting biomolecule

Abstract

Provided is a method of sensing biomolecules using a bioFET, the method including: forming a layer including Au on a gate of the bioFET; forming a probe immobilized on a substrate separated from the gate by a predetermined distance, and a biomolecule having a thiol group (—SH) which is incompletely bonded to the probe; reacting the probe with a sample including a target molecule; and measuring a current flowing in a channel region between a source and a drain of the bioFET.

1 . A method of sensing biomolecules in an electrolyte using a bioFET, the method comprising:
 (a) immobilizing a first probe on a layer comprising Au on a gate of the bioFET;   (b) supplying a second probe to which a liposome containing a thiol group (—SH) compound is bonded;   (c) reacting a target molecule-containing sample with the first probe and the second probe, and then washing the result;   (d) bursting the liposome; and   (e) measuring a current flowing in a channel region between a source and drain of the bioFET.   
     
     
         2 . The method of  claim 1 , wherein the target is DNA, RNA, or a protein. 
     
     
         3 . The method of  claim 1 , wherein a portion of the target complementarily corresponds to the first probe and another portion of the target complementarily corresponds to the second probe. 
     
     
         4 . The method of  claim 1 , wherein the thiol group compound is one of mercaptohexanol and cysteine. 
     
     
         5 . The method of  claim 1 , wherein, in operation (d), the liposome is burst due to a gradient in osmotic pressure. 
     
     
         6 . The method of  claim 1 , wherein the flow of the current in operation (e) is generated by bonding of a thiol group, which is released by the burst of the liposome in operation (d), with Au formed on the surface of the gate.