Method for Promoting Wound Healing

Abstract

The present invention discloses a method for treating wounds and for accelerating the healing of wounds by administering an effective amount of a pharmaceutical composition containing type VII collagen protein, mini-C7 protein, variants thereof or any combinations thereof. The pharmaceutical composition may be administered through a variety of routes including intravenous injection, topical application, or oral ingestion. The method may further include administering a genetically modified fibroblast capable of expressing type VII collagen protein, mini-C7 protein, variants thereof or small growth factors to achieve synergistic healing effect.

1 . A method for treating or accelerating the healing of a wound in a subject, comprising:
 Administering directly into a subject in need thereof an effective amount of a pharmaceutical composition comprising C7, mini-C7, or a variant thereof and a gene-corrected RDEB fibroblast, a lentiviral vector expressing C7, or a variant thereof, wherein said wound is caused by a defect in the gene that encodes for type VII collagen.   
   
   
       2 . The method of  claim 1 , wherein said administration topical application, intravenous injection, intramuscular injection, inhalation, oral ingestion or combinations thereof. 
   
   
       3 . The method of  claim 1 , wherein said gene-corrected RDEB fibroblast synthesizes and expresses one or more of C7, mini-C7, type I collagen, type III collagen, type XVII collagen, small growth factors, or combinations thereof. 
   
   
       4 . (canceled) 
   
   
       5 . (canceled) 
   
   
       6 . The method of  claim 3 , wherein said small growth factor is epidermal growth factor (EGF), transforming growth factor alpha (TGF alpha), transforming growth factor beta (TGF beta), platelet derived growth factor (PDGF), or a combination thereof.