Chimeric CrylE Delta Endotoxin and Methods of Controlling Insects

Abstract

The present invention relates to a chimeric δ endotoxin protein Cry 1E of SEQ ID No. 1 with extraordinarily high insecticidal property and a chimera gene of SEQ ID No. 2 encoding the said chimeric protein, and also a method of treating insect infested plants using said chimera protein.

1 - 41 . (canceled) 
     
     
         42 . A chimeric δ-endotoxin protein comprising an amino acid sequence that is identical to amino acids 530-641 of SEQ ID NO:1, said chimeric protein having greater toxicity than Cry1C as determined by EC 50  in a challenge to neonatal  Spodoptera litura  larvae. 
     
     
         43 . A chimeric δ-endotoxin protein comprising an amino acid sequence that is at least 95% identical to amino acids 1-641 of SEQ ID NO:1, said protein having a sequence identical to amino acids 530-641 of SEQ ID NO:1, said chimeric protein having greater toxicity than Cry1C as determined by EC 50  in a challenge to neonatal  Spodoptera litura  larvae. 
     
     
         44 . The protein as claimed in  claim 42  or  43 , wherein said chimeric protein is stable at temperature ranging between 4-35° C.