Method of culturing pluripotent stem cells using extracellular matrix from fetal membrane-derived cells

Abstract

Provided are a method of culturing pluripotent stem cells in the presence of a decidua-derived cell or an extracellular matrix derived from the cell, that enables safe and efficient maintenance culture and derivation of pluripotent stem cells; a culture agent for pluripotent stem cells, that comprises a decidua-derived cell or an extracellular matrix derived from the cell; and other means for developing or performing the method.

1 . A method of culturing pluripotent stem cells in the presence of decidua-derived cells or an extracellular matrix derived from the cell. 
     
     
         2 . The method according to  claim 1 , wherein the decidua-derived cell is a mesenchymal cell. 
     
     
         3 . The method according to  claim 1 , wherein both the decidua-derived cell and the pluripotent stem cells are derived from a mammal of the same species. 
     
     
         4 . The method according to  claim 3 , wherein the mammal is a human. 
     
     
         5 . The method according to  claim 1 , wherein the pluripotent stem cells are human ES cells or human iPS cells. 
     
     
         6 . A culture agent for pluripotent stem cells, containing decidua-derived cells or an extracellular matrix derived from the cell. 
     
     
         7 . The agent according to  claim 6 , wherein the decidua-derived cell is a mesenchymal cell. 
     
     
         8 . The agent according to  claim 6 , wherein both the decidua-derived cell and the pluripotent stem cells are derived from a mammal of the same species. 
     
     
         9 . The agent according to  claim 8 , wherein the mammal is a human. 
     
     
         10 . The agent according to  claim 6 , wherein the pluripotent stem cells are human ES cells or human iPS cells. 
     
     
         11 . A container for culturing pluripotent stem cells, coated with an extracellular matrix from decidua-derived cells. 
     
     
         12 . The container according to  claim 11 , wherein the decidua-derived cell is a mesenchymal cell. 
     
     
         13 . The container according to  claim 11 , wherein both the decidua-derived cell and the pluripotent stem cells are derived from a mammal of the same species. 
     
     
         14 . The container according to  claim 13 , wherein the mammal is a human. 
     
     
         15 . The container according to  claim 11 , wherein the pluripotent stem cells are human ES cells or human iPS cells. 
     
     
         16 . A kit for culturing pluripotent stem cells, comprising (i) and (ii) below:
 (i) a culture agent for pluripotent stem cells, comprising decidua-derived cells or an extracellular matrix derived from the cell; and   (ii) an explanatory document stating that the kit should be used, or can be used, for culturing pluripotent stem cells.   
     
     
         17 . A kit for culturing pluripotent stem cells, comprising (i) and (ii) below:
 (i) a container for culturing pluripotent stem cells, coated with an extracellular matrix from decidua-derived cells; and   (ii) an explanatory document stating that the kit should be used, or can be used, for culturing pluripotent stem cells.