Yield Increase in Plants Overexpressing the SHSRP Genes

Abstract

A transgenic crop plant transformed by a Serine Hydroxymethyltransferase-Like Stress-Related Polypeptide (SHSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the crop plant results in the plant's increased root growth, and/or increased yield, and/or increased tolerance to environmental stress as compared to a wild type variety of the plant Also provided are agricultural products, including seeds, produced by the transgenic crop plants. Also provided are isolated novel SHSRPs, and isolated novel nucleic acids encoding SHSRPs, and vectors and transgenic plant containing the same.

1 . A transgenic crop plant transformed with an isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
 a) a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;   b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2;   c) a polynucleotide having at least 70% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;   d) a polynucleotide encoding a polypeptide having at least 70% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2; and   e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above.   
     
     
         2 . The transgenic crop plant of  claim 1 , wherein the expression of the polynucleotide in the plant results in increased yield under normal or stress conditions as compared to a wild type variety of the plant. 
     
     
         3 . The transgenic crop plant of  claim 1 , wherein the expression of the polynucleotide in the plant results in increased stress tolerance to an environmental stress as compared to a wild type variety of the plant. 
     
     
         4 . The transgenic crop plant of  claim 1 , wherein the expression of the polynucleotide in the plant results in increased root growth under normal or stress conditions as compared to a wild type variety of the plant. 
     
     
         5 . The transgenic crop plant of  claim 1 , wherein the plant is a monocot. 
     
     
         6 . The transgenic crop plant of  claim 1 , wherein the plant is a dicot. 
     
     
         7 . The transgenic crop plant of  claim 1 , wherein the plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, sorghum, millet, sugarcane, soybean, peanut, cotton, rapeseed, canola,  manihot , pepper, sunflower, tagetes, solanaceous plants, potato, tobacco, eggplant, tomato,  Vicia  species, pea, alfalfa, coffee, cacao, tea,  Salix  species, oil palm, coconut, perennial grass and a forage crop plant. 
     
     
         8 . The transgenic crop plant of  claim 1 , wherein the plant is a whole plant, a plant cell, a plant part, or a plant seed. 
     
     
         9 . A crop plant seed produced by the transgenic crop plant of  claim 1 , wherein the seed comprises the isolated nucleic acid. 
     
     
         10 . The seed of  claim 9 , wherein the seed is true breeding for increased yield under normal or stress conditions as compared to a wild type variety of the seed. 
     
     
         11 . The seed of  claim 9 , wherein the seed is true breeding for increased stress tolerance to an environmental stress as compared to a wild type variety of the seed. 
     
     
         12 . The seed of  claim 9 , wherein the seed is true breeding for an increased root growth under normal or stress conditions as compared to a wild type variety of the seed. 
     
     
         13 . A method of producing a transgenic crop plant containing an isolated nucleic acid encoding a polypeptide, wherein the method comprises the steps of, transforming a plant cell with an expression vector comprising the nucleic acid, and generating from the plant cell the transgenic plant that expresses the polypeptide, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
 a) a polynucleotide having a sequence as set forth in any of SEQ ED NOS as provided in Column No. 3 of Table 1 and Table 2;   b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ D NOS as provided in Column No. 4 of Table 1 and Table 2;   c) a polynucleotide having at least 70% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;   d) a polynucleotide encoding a polypeptide having at least 70% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2; and   e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above.   
     
     
         14 . The method of  claim 13 , wherein the expression of the polynucleotide in the plant results in increased yield under normal or stress conditions as compared to a wild type variety of the plant. 
     
     
         15 . The method of  claim 13 , wherein the expression of the polynucleotide in the plant results in increased stress tolerance to an environmental stress as compared to a wild type variety of the plant. 
     
     
         16 . The method of  claim 13 , wherein the expression of the polynucleotide in the plant results in increased root growth under normal or stress conditions as compared to a wild type variety of the plant. 
     
     
         17 . The method of  claim 13 , wherein the crop plant is a monocot. 
     
     
         18 . The method of  claim 13 , wherein the crop plant is a dicot. 
     
     
         19 . The method of  claim 13 , wherein the crop plant is selected from the group consisting of maize, wheat, rye, oat, triticale, rice, barley, sorghum, millet, sugarcane, soybean, peanut, cotton, rapeseed, canola,  manihot , pepper, sunflower, tagetes, solanaceous plants, potato, tobacco, eggplant, tomato,  Vicia  species, pea, alfalfa, coffee, cacao, tea,  Salix  species, oil palm, coconut, perennial grass and a forage crop plant. 
     
     
         20 . The method of  claim 13 , wherein the nucleic acid comprises a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2. 
     
     
         21 . The method of  claim 13 , wherein the nucleic acid comprises a polynucleotide having at least 70% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2. 
     
     
         22 . The method of  claim 13 , wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2. 
     
     
         23 . The method of  claim 13 , wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 70% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2. 
     
     
         24 . The method of  claim 13 , wherein the nucleic acid is operably linked to one or more regulatory sequences. 
     
     
         25 . The method of  claim 24 , wherein the regulatory sequence is a promoter. 
     
     
         26 . The method of  claim 25 , wherein the promoter is tissue specific. 
     
     
         27 . The method of  claim 25 , wherein the promoter is developmentally regulated. 
     
     
         28 . An isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
 a) a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2;   b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2;   c) a polynucleotide having at least 90% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2;   d) a polynucleotide encoding a polypeptide having at least 92% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2;   e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above; and   f) a polynucleotide complementary to any of the polynucleotides of a) through d) above.   
     
     
         29 . The isolated nucleic acid of  claim 28 , wherein the nucleic acid comprises a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2. 
     
     
         30 . The isolated nucleic acid of  claim 28 , wherein the nucleic acid comprises a polynucleotide having at least 90% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2. 
     
     
         31 . The isolated nucleic acid of  claim 28 , wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2. 
     
     
         32 . The isolated nucleic acid of  claim 28 , wherein the nucleic acid comprises a polynucleotide encoding a polypeptide having at least 92% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2. 
     
     
         33 . A transgenic plant transformed with an isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
 a) a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2;   b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2;   c) a polynucleotide having at least 90% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2;   d) a polynucleotide encoding a polypeptide having at least 92% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2; and   e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above.   
     
     
         34 . The transgenic plant of  claim 33 , wherein expression of the polynucleotide in the plant results in increased yield under normal or stress conditions as compared to a wild type variety of the plant. 
     
     
         35 . The transgenic plant of  claim 33 , wherein expression of the polynucleotide in the plant results in increased stress tolerance to an environmental stress as compared to a wild type variety of the plant. 
     
     
         36 . The transgenic plant of  claim 33 , wherein expression of the polynucleotide in the plant results in increased root growth under normal or stress conditions as compared to a wild type variety of the plant. 
     
     
         37 . The transgenic plant of  claim 33 , wherein the plant is a whole plant, a plant cell, a plant part, or a plant seed. 
     
     
         38 . A plant seed produced by the transgenic plant of  claim 33 , wherein the seed comprises the isolated nucleic acid. 
     
     
         39 . The seed of  claim 38 , wherein the seed is true breeding for increased yield under normal or stress conditions as compared to a wild type variety of the seed. 
     
     
         40 . The seed of  claim 38 , wherein the seed is true breeding for increased stress tolerance to an environmental stress as compared to a wild type variety of the seed. 
     
     
         41 . The seed of  claim 3 S, wherein the seed is true breeding for an increased root growth under normal or stress conditions as compared to a wild type variety of the seed. 
     
     
         42 . The transgenic plant of  claim 33 , wherein the plant is a monocot. 
     
     
         43 . The transgenic plant of  claim 33 , wherein the plant is a dicot. 
     
     
         44 . A recombinant expression vector comprising an isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
 a) a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;   b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ D NOS as provided in Column No. 4 of Table 1 and Table 2;   c) a polynucleotide having at least 90% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;   d) a polynucleotide encoding a polypeptide having at least 92% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2;   e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above; and   f) a polynucleotide complementary to any of the polynucleotides of a) through d) above.   
     
     
         45 . The recombinant expression vector of  claim 44 , wherein the vector further comprises one or more regulatory sequences. 
     
     
         46 . The recombinant expression vector of  claim 45 , wherein the regulatory sequence is a promoter. 
     
     
         47 . The recombinant expression vector of  claim 46 , wherein the promoter is tissue specific. 
     
     
         48 . The recombinant expression vector of  claim 46 , wherein the promoter is developmentally regulated.