Anti-bacterial plant compositions

Abstract

The invention provides a pharmaceutical composition which comprises a pharmaceutical carrier and at least one compound selected from the group consisting of cobaltocene-octamethyl, stigmastan-3,5-diene, galoxolide, benzyl salicylate, eucalyptol, and α-pinene. Also provided is a method of preparing a composition having antimicrobial activity comprising extracting a plant material in an organic solvent, contacting the extracted material to a chromatographic column, and eluting from the chromatographic column with a mobile polar phase to obtain a composition. The plant material is from Mammea Americana, Marchntia polymorpha , or Callistemon citrinus . Also provided is a method of inhibiting the growth of a mycobacterium, comprising administering a composition comprising a carrier and at least one compound selected from among cobaltocene-octamethyl, stigmastan, 3,5-diene, friedelin, galoxolide, benzyl salicylate, eucalyptol, and α-pinene.

1 . A pharmaceutical composition comprising a pharmaceutical carrier and cobaltocene-octamethyl in isolated or purified form, wherein the composition has antimicrobial activity. 
   
   
       2 . The pharmaceutical composition of  claim 1  further comprising at least one compound selected from the group consisting of a caryophylene, friedelin, cyclododecane, β-myrceane, acetic acid, and a terpene. 
   
   
       3 . The pharmaceutical composition of  claim 1  further comprising friedelin. 
   
   
       4 . The pharmaceutical composition of  claim 1  further comprising stigmastan-3,5,-diene. 
   
   
       5 . The pharmaceutical composition of  claim 1  further comprising a terpene. 
   
   
       6 . The pharmaceutical composition of  claim 1  further comprising a caryophylene. 
   
   
       7 . The pharmaceutical composition of  claim 1  further comprising β-myrceane. 
   
   
       8 . The pharmaceutical composition of  claim 1  further comprising acetic acid. 
   
   
       9 . The pharmaceutical composition of  claim 1 , wherein the cobaltocene octamethyl is cobaltocene 1,1′,2,2′,3,3′,4,4′-octamethyl. 
   
   
       10 . The pharmaceutical composition of  claim 1 , wherein the pharmaceutical composition has antibacterial activity. 
   
   
       11 . The pharmaceutical composition of  claim 10 , wherein the antibacterial activity is against a mycobacterium. 
   
   
       12 . The pharmaceutical composition of  claim 11 , wherein the mycobacterium is  M. avium, M. bovis, M. intracellulare, M. kansaii, M. leprae, M. marinum, M. phlei, M. scrofulaceum, M. smegmatis, M. fortuitum, M. tuberculosis , or  M. ulcerans.