Cascade

Abstract

The invention provides the use of an enzyme and a prodrug in the manufacture of a medicament for use in inducing an anti-tumour immune response in a human patient.

1 . A method for inducing an anti-tumor immune response in a subject, comprising administering an enzyme and prodrug therapy to the subject, wherein the enzyme and prodrug therapy is directed against a first tumor in the subject, thereby inducing an anti-tumor immune response to a second tumor in the subject. 
     
     
         2 . The method according to  claim 1 , wherein the enzyme is cytochrome p450 and the prodrug is oral oxazaphosphorine. 
     
     
         3 . The method according to  claim 2 , wherein the oxazaphosphorine is cyclophosphamide or ifosphamide. 
     
     
         4 . The method according to  claim 1 , wherein a nucleotide sequence encodes the enzyme. 
     
     
         5 . The method according to  claim 1 , wherein the anti-tumor immune response is an antibody response directed to a tumor antigen. 
     
     
         6 . The method according to  claim 1 , wherein the anti-tumor immune response is a T-cell response. 
     
     
         7 . The method according to  claim 1 , wherein the anti-tumor immune response is a combined T-cell and antibody response. 
     
     
         8 . The method according to  claim 1 , further comprising administering an immune stimulatory molecule. 
     
     
         9 . The method according to  claim 1 , further comprising detecting anti-tumor antigen antibodies. 
     
     
         10 - 16 . (canceled)