Novel bacteria and uses thereof

Abstract

The present invention relates to a novel bacterium Yersinia entomophaga MH96 as deposited at DSMZ on 4 May 2006 and designated accession no. DSM 18238. The present invention also relates to substances obtained or derived from Yersinia entomophaga MH96, which retain biopesticide activity. Methods for protecting a plant from pest infestation which include applying to the plant or its environment an effective amount of Yersinia entomophaga MH96 or a product delivered from the bacterium are also described.

1 . An isolated  Yersinia entomophaga  MH96 bacterium deposited at DSMZ on 4 May 2006 and designated accession no. DSM 18238. 
   
   
       2 . The use of an isolated  Yersinia entomophaga  MH96 bacterium as claimed in  claim 1 , to directly or indirectly obtain a biopesticide. 
   
   
       3 . The use of  Yersinia entomophaga  MH96 bacterium as claimed in  claim 1 , as a biopesticide. 
   
   
       4 . The use of  Yersinia entomophaga  MH96 bacterium as claimed in  claim 1 , in the manufacture of a composition suitable as a biopesticide. 
   
   
       5 . A culture of  Yersinia entomophaga  MH96 bacterium as deposited at DSMZ on 4 May 2006 and designated accession no. DSM 18238 bacterium. 
   
   
       6 . The use of a culture of  Yersinia entomophaga  MH96 as claimed in  claim 5 , to directly or indirectly obtain a biopesticide. 
   
   
       7 . The use of a culture of  Yersinia entomophaga  MH96 as claimed in  claim 5 , as a biopesticide. 
   
   
       8 . The use of a culture of  Yersinia entomophaga  MH96 as claimed in  claim 5 , in the manufacture of a composition suitable as a biopesticide. 
   
   
       9 . A cellular extract obtained from  Yersinia entomophaga  MH96 deposited at DSMZ on 4 May 2006 and designated accession no. DSM 18238 or a culture thereof. 
   
   
       10 . The use of a cellular extract of  Yersinia entomophaga  MH96 as claimed in  claim 9 , to directly or indirectly obtain a biopesticide. 
   
   
       11 . The use of a cellular extract of  Yersinia entomophaga  MH96 as claimed in  claim 9 , as a biopesticide. 
   
   
       12 . The use of a cellular extract of  Yersinia entomophaga  MH96 as claimed in  claim 9 , in the manufacture of a composition suitable as a biopesticide. 
   
   
       13 . A sonicated cell filtrate of  Yersinia entomophaga  MH96 as deposited at DSMZ on 4 May 2006 and designated accession no. DSM 18238 which has a biopesticide activity. 
   
   
       14 . The use of a sonicated cell filtrate of  Yersinia entomophaga  MH96 as claimed in  claim 13 , to directly or indirectly obtain a biopesticide. 
   
   
       15 . The use of a sonicated cell filtrate of  Yersinia entomophaga  MH96 as claimed in  claim 13 , as a biopesticide. 
   
   
       16 . The use of a sonicated cell filtrate of  Yersinia entomophaga  MH96 as claimed in  claim 13 , in the manufacture of a composition suitable as a biopesticide. 
   
   
       17 . A supernatant of a whole broth culture of  Yersinia entomophaga  MH96 as deposited at DSMZ on 4 May 2006 and designated accession no. DSM 18238. 
   
   
       18 . The use of the supernatant of a whole broth culture of  Yersinia entomophaga  MH96 as claimed in  claim 17 , as a biopesticide. 
   
   
       19 . The use of the supernatant of a whole broth culture of  Yersinia entomophaga  MH96 as claimed in  claim 17 , to directly or indirectly obtain a biopesticide. 
   
   
       20 . The use of the supernatant of a whole broth culture of  Yersinia entomophaga  MH96 as claimed in  claim 17 , in the manufacture of a composition suitable as a biopesticide. 
   
   
       21 . A composition that includes  Yersinia entomophaga  MH96, wherein said bacteria exhibits a biopesticide activity. 
   
   
       22 . A composition as claimed in  claim 21 , wherein the composition is formulated from a culture of  Yersinia entomophaga  MH96 wherein said culture exhibits a biopesticide activity. 
   
   
       23 . A composition as claimed in  claim 21 , wherein the composition is formulated from a cellular extract of  Yersinia entomophaga  MH96, wherein said extract exhibits a biopesticide activity. 
   
   
       24 . A composition as claimed in  claim 21 , wherein the composition is formulated from a sonciated cell filtrate of  Yersinia entomophaga  MH96, wherein said extract exhibits a biopesticide activity. 
   
   
       25 . A composition as claimed in  claim 21 , wherein the composition is formulated from a whole broth culture of  Yersinia entomophaga  MH96 wherein said whole broth culture exhibits a biopesticide activity. 
   
   
       26 . A composition as claimed in  claim 21 , wherein the composition is formulated from a supernatant of a whole broth culture of  Yersinia entomophaga  MH96 wherein the supernatant from the culture exhibits a biopesticide activity. 
   
   
       27 . A composition including an effective amount  Yersinia entomophaga  MH96, wherein  Yersinia entomophaga  MH96 has been killed as an intact form and maintains a biopesticide activity. 
   
   
       28 . A composition as claimed in any one of  claims 21  to  27 , wherein the composition is formulated with at least one biopolymer compound. 
   
   
       29 . A composition as claimed in  claim 28 , wherein the at least one biopolymer compound is at least one type of gum compound. 
   
   
       30 . A composition as claimed in  claim 28  or  claim 29 , wherein the formulated composition is a gel. 
   
   
       31 . A composition as claimed in any one of  claims 21  to  27 , wherein the composition is formulated with at least one biopolymer compound and at least one desiccating agent. 
   
   
       32 . A composition as claimed in  claim 31 , wherein the at least one biopolymer compound is at least one type of gum compound and the at least one desiccating agent is at least one inert clay compound. 
   
   
       33 . A composition as claimed in  claim 31  or  claim 32 , wherein the formulated composition is a dough or granular material. 
   
   
       34 . A composition as claimed in any one of  claims 28  to  33 , wherein the composition is formed into a prill or granule shape. 
   
   
       35 . A composition as claimed in any one of  claims 28  to  33 , wherein on application, the composition is mixed with an aqueous liquid and sprayed onto a substrate. 
   
   
       36 . A composition as claimed in any one of  claims 28  to  34 , wherein the composition is coated onto a substrate. 
   
   
       37 . A composition as claimed in  claim 36 , wherein the substrate is a seed. 
   
   
       38 . A method of treating or protecting a plant and/or plant derived materials from pest infestation wherein the method comprises applying to the plant or its environment an effective amount of  Yersinia entomophaga  MH96. 
   
   
       39 . A method as claimed in  claim 38 , wherein the effective amount of  Yersinia entomophaga  MH96 is obtained from a culture of  Yersinia entomophaga  MH96. 
   
   
       40 . A method as claimed in  claim 38 , wherein the effective amount of  Yersinia entomophaga  MH96 is obtained from a supernatant from a whole broth culture of  Yersinia entomophaga  MH96. 
   
   
       41 . A method of treating or protecting a plant and/or plant derived materials from pest infestation wherein the method comprises applying to the plant or its environment an effective amount of a cellular extract of  Yersinia entomophaga  MH96. 
   
   
       42 . A method of treating or protecting a plant and/or plant derived materials from pest infestation wherein the method comprises applying to the plant or its environment an effective amount of a sonicated cell filtrate of  Yersinia entomophaga  MH96. 
   
   
       43 . A method of treating or protecting a plant and/or plant derived materials from pest infestation wherein the method comprises applying to the plant or its environment a composition comprising an effective amount of  Yersinia entomophaga  MH96. 
   
   
       44 . A method as claimed in  claim 43 , wherein the effective amount of  Yersinia entomophaga  MH96 is obtained from a culture of  Yersinia entomophaga  MH96 bacterium. 
   
   
       45 . A method as claimed in  claim 43 , wherein the effective amount of  Yersinia entomophaga  MH96 is obtained from a supernatant from a whole broth culture  Yersinia entomophaga  MH96. 
   
   
       46 . A method of treating or protecting a plant and/or plant derived materials from pest infestation wherein the method comprises applying to the plant or its environment a composition comprising an effective amount of a cellular extract of  Yersinia entomophaga  MH96 bacterium. 
   
   
       47 . A method of treating or protecting a plant and/or plant derived materials from pest infestation wherein the method comprises applying to the plant or its environment a composition comprising an effective amount of a sonicated cell filtrate of  Yersinia entomophaga  MH96. 
   
   
       48 . A method of controlling and/or preventing a pest infestation characterised by the step of applying a composition comprising an effective amount of  Yersinia entomophaga  MH96 to a surface. 
   
   
       49 . A method as claimed in  claim 48 , wherein the effective amount of  Yersinia entomophaga  MH96 is obtained from a culture of  Yersinia entomophaga  MH96. 
   
   
       50 . A method as claimed in  claim 48 , wherein the effective amount of  Yersinia entomophaga  MH96 is obtained from a supernatant from a whole broth culture of  Yersinia entomophaga  MH96. 
   
   
       51 . A method of controlling and/or preventing a pest infestation characterised by the step of applying a composition comprising an effective amount of a cellular extract of  Yersinia entomophaga  MH96 to a surface. 
   
   
       52 . A method of controlling and/or preventing a pest infestation characterised by the step of applying a composition comprising an effective amount of a sonicated cell filtrate of  Yersinia entomophaga  MH96 to a surface. 
   
   
       53 . A method of controlling and/or preventing a pest infestation characterised by the step of applying a composition as claimed in any one of  claims 21  to  37 , to a surface.