Bacterium producing L-glutamic acid and method for producing L-glutamic acid

Abstract

L-Glutamic acid is produced by culturing a coryneform bacterium having L-glutamic acid producing ability, in which trehalose synthesis ability is decreased or deleted by, for example, disrupting a gene coding for trehalose-6-phosphate synthase, a gene coding for maltooligosyltrehalose synthase, or both of these genes to produce and accumulate L-glutamic acid in the medium, and collecting the L-glutamic acid from the medium.

1 - 7 . (canceled) 
     
     
         8 . An isolated DNA coding for a protein defined in the following (A) or (B):
 (A) a protein having the amino acid sequence of SEQ ID NO: 32,   (B) a protein having the amino acid sequence of SEQ ID NO: 32 including substitution, deletion, insertion or addition of 1-20 amino acid residues and having maltooligosyltrehalose synthase activity.   
     
     
         9 . The isolated DNA according to  claim 8 , which is a DNA defined in the following (a) or (b):
 (a) a DNA comprising at least the nucleotide residues 82-2514 in the nucleotide sequence of SEQ ID NO: 31, or   (b) a DNA which is hybridizable with a nucleotide sequence complementary to the nucleotide sequence comprising at least the nucleotide residues 82-2514 in the nucleotide sequence of SEQ ID NO: 31 under a stringent condition, and which codes for a protein having maltooligosyltrehalose synthase activity, wherein the stringent condition is 1×SSC, 0.1% SDS, at 60° C.