Bisulfite Conversion Reagent

Abstract

Disclosed, among other things, are packaged bisulfite solutions comprising bisulfite reagent in an oxygen-impermeable container and methods.

1 ) A packaged bisulfite solution comprising an aqueous deoxygenated solution of a bisulfite reagent enclosed within an oxygen-impermeable container, wherein after six months, the solution has a bisulfite concentration that is at least 90% of the original concentration. 
   
   
       2 ) The packaged bisulfite solution of  claim 1 , wherein the bisulfite reagent comprises at least one counter-ion selected from a monovalent ion, a divalent ion and combinations thereof. 
   
   
       3 ) The packaged bisulfite solution of  claim 2 , wherein the counter-ion is a monovalent ion. 
   
   
       4 ) The packaged bisulfite solution of  claim 2 , wherein the counter-ion is a monovalent ion selected from sodium, lithium, potassium, ammonium, tetraalkylammonium and combinations thereof. 
   
   
       5 ) The packaged bisulfite solution of  claim 4 , wherein the counter-ion is sodium. 
   
   
       6 ) The packaged bisulfite solution of  claim 4 , wherein the counter-ion is lithium or potassium. 
   
   
       7 ) The packaged bisulfite solution of  claim 4 , wherein the counter-ion is ammonium. 
   
   
       8 ) The packaged bisulfite solution of  claim 4 , wherein the counter-ion is tetraalkylammonium. 
   
   
       9 ) The packaged bisulfite of  claim 2 , wherein the counter-ion is a divalent ion. 
   
   
       10 ) The packaged bisulfite solution of  claim 2 , wherein the counter-ion is a divalent ion selected from magnesium, manganese, calcium and combinations thereof. 
   
   
       11 ) The packaged bisulfite solution of  claim 10 , wherein the counter-ion is magnesium. 
   
   
       12 ) The packaged bisulfite solution of  claim 10 , wherein the counter-ion is manganese. 
   
   
       13 ) The packaged bisulfite solution of  claim 10 , wherein the counter-ion is calcium. 
   
   
       14 ) The packaged bisulfite solution according to any one of the preceding claims, wherein the oxygen-impermeable container is selected from an ampoule, a hermetically sealed bag, a capsule, etc. 
   
   
       15 ) The packaged bisulfite solution of  claim 14 , wherein the oxygen-impermeable container is an ampoule. 
   
   
       16 ) The packaged bisulfite solution of  claim 15 , wherein the ampoule is an amber glass ampoule. 
   
   
       17 ) The packaged bisulfite solution of  claim 15 , wherein the oxygen-impermeable container is a capsule. 
   
   
       18 ) The packaged bisulfite solution of  claim 17 , wherein the capsule comprises a substantially oxygen-impermeable thermal plastic polymer. 
   
   
       19 ) The packaged bisulfite solution according to any one of the preceding claims, wherein the bisulfite solution contains less than 10 ppm dissolved oxygen. 
   
   
       20 ) A kit comprising the packaged bisulfite solution according to  claim 1 .