Subtilisin variants

Abstract

New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.

1 . An isolated or recombinant polypeptide with an improved endo-protease activity selected from among increased thermotolerance, increased activity at ambient temperature, increased activity at alkaline pH, increased activity at acid pH, increased activity at neutral pH, and increased activity in the presence of organic solvents, relative to the subtilisin homologue polypeptide corresponding to SEQ ID NO: 261, which polypeptide has at least 70% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260, over a comparison window of at least 20 contiguous amino acids. 
     
     
         2 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide has at least 80% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         3 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide has at least 85% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         4 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide has at least 90% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         5 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide has at least 95% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         6 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide has at least 96% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         7 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide has at least 97% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         8 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide has at least 98% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         9 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide has at least 99% sequence identity to at least one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         10 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide comprises an amino acid sequence of any one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 20 contiguous amino acids. 
     
     
         11 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide comprises an amino acid sequence of any one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 30 contiguous amino acids. 
     
     
         12 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide comprises an amino acid sequence of any one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 50 contiguous amino acids. 
     
     
         13 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide comprises an amino acid sequence of any one of SEQ ID NO: 131 to SEQ ID NO: 260 over a comparison window of at least 100 contiguous amino acids. 
     
     
         14 . The isolated or recombinant polypeptide of  claim 1 , which polypeptide comprises an amino acid sequence of any one of SEQ ID NO: 131 to SEQ ID NO: 260. 
     
     
         15 - 45 . (canceled) 
     
     
         46 . A composition comprising the polypeptide of  claim 1  and a detergent. 
     
     
         47 - 123 . (canceled)