Icbp90 polypeptide and its fragments and polynucleotides coding for said polypeptides and applications for diagnosing and treating cancer

Abstract

The invention concerns a novel ICBP90 (Inverted CCAAT box binding protein 90) and its fragments, polynucleotides coding for said polypeptides and specific antibodies directed against said polypeptides. The invention also concerns methods and kits for diagnosing cell proliferation and compounds useful as medicine for preventing and/or treating pathology involving cell proliferation and in particular cancer.

1 - 39 . (canceled) 
     
     
         40 . An isolated monoclonal or polyclonal antibody and its fragments, which specifically binds a first polypeptide that a) comprises the amino acid sequence SEQ ID NO. 2 or b) consists of a fragment of the amino acid sequence SEQ ID NO. 2, wherein said fragment comprises amino acids 263-793 of the amino acid sequence SEQ ID NO. 2. 
     
     
         41 . The monoclonal antibody according to  claim 40 , wherein said antibody is specific for the first polypeptide and is capable of inhibiting the interaction between the first polypeptide and a DNA sequence, to which the first polypeptide is specifically bound and wherein said DNA sequence is inverted CCAAT box ICB1 or inverted CCAAT box ICB2. 
     
     
         42 . The monoclonal antibody according to  claim 40 , wherein said antibody is specific for the first polypeptide and is capable of inhibiting an interaction between the first polypeptide and a second polypeptide, said second polypeptide i) comprises the amino acid sequence SEQ ID NO. 2 or ii) is a protein of a transcriptional complex. 
     
     
         43 . A method for detecting and/or measuring a polypeptide, that a) comprises the amino acid sequence SEQ ID NO. 2 or b) consists of a fragment of the amino acid sequence SEQ ID NO. 2, wherein said fragment comprises amino acids 263-793 of the amino acid sequence SEQ ID NO. 2, in a biological sample, comprising:
 a) contacting the biological sample with the monoclonal or polyclonal antibody of  claim 40 ; and   b) revealing a formed antigen-antibody complex.   
     
     
         44 . A kit for detecting and/or measuring a polypeptide that a) comprises the amino acid sequence SEQ ID NO. 2 or b) consists of a fragment of the amino acid sequence SEQ ID NO. 2, wherein said fragment comprises amino acids 263-793 of the amino acid sequence SEQ ID NO. 2, in a biological sample by immunological reaction, comprising:
 a) the isolated antibody of  claim 40 ;   b) if applicable, medium reagents for formation of a medium for the immunological reaction; and   c) detection reagents enabling detection of the antigen-antibody complex produced by the immunological reaction.   
     
     
         45 . A composition for detecting, localizing and imaging cancers, comprising the isolated antibody according to  claim 40 , wherein the antibody is labeled directly or indirectly with a marker generating a signal, said marker is selected from the group consisting of radioactive isotopes and nonisotope entities. 
     
     
         46 . A method for detecting, localizing and imaging cancer cells, comprising:
 a) injecting parenterally the composition according to  claim 45  in a human being;   b) penetrating the labeled antibody within said cancer cells, without said antibody being bound substantially to normal cells;   c) detecting a signal from the labeled antibody by means of a signal detector; and   d) converting the detected signal into an image of cancer cells.   
     
     
         47 . A method for detecting and/or measuring a polypeptide that a) comprises the amino acid sequence SEQ ID NO. 2 or b) consists of a fragment of the amino acid sequence SEQ ID NO. 2, wherein said fragment comprises amino acids 263-793 of the amino acid sequence SEQ ID NO. 2, a biological sample, comprising:
 a) contacting the biological sample with an antibody, which is specific to the polypeptide and is capable of inhibiting an interaction between the polypeptide and a DNA sequence, to which the polypeptide is specifically bound;   b) and revealing a formed antigen-antibody complex.   
     
     
         48 . A method for detecting and/or measuring a polypeptide according that a) comprises the amino acid sequence SEQ ID NO. 2 or b) consists of a fragment of the amino acid sequence SEQ ID NO. 2, wherein said fragment comprises amino acids 263-793 of the amino acid sequence SEQ ID NO. 2, in a biological sample, comprising:
 a) contacting the biological sample with an antibody, which is specific to the polypeptide and is capable of inhibiting an interaction between the polypeptide and a protein, with which the polypeptide interacts, said protein i) comprises the amino acid sequence SEQ ID NO. 2 or ii) is a protein of a transcriptional complex;   b) and revealing a formed antigen-antibody complex.