High affinity anti-n1,n12-diacetylspermine monoclonal antibody

Abstract

The present invention provides an anti-diacetylspermine specific monoclonal antibody which satisfies a specific measuring condition in an immunoreaction system between a solid-phased diacetylspermine and the anti-diacetylspermine specific monoclonal antibody, wherein the immunoreaction system comprises a sample diacetylspermine or sample N 1 -acetylspermidine to inhibit the immunoreaction; and the specific measuring condition is that the 50% inhibition activity against the immunoreaction by the diacetylspermine in the sample is at least 1,000 times as much as the 50% inhibition activity against the immunoreaction by the N 1 -acetylspermidine in the sample.

1 . An anti-diacetylspermine specific monoclonal antibody which satisfies a specific measuring condition in an immunoreaction system between a solid-phased diacetylspermine and the anti-diacetylspermine specific monoclonal antibody, Wherein said system comprises a sample diacetylspermine or sample N 1 -acetylspermidine to inhibit said immunoreaction; and said specific measuring condition is that the 50% inhibition activity against said immunoreaction by the diacetylspermine in the sample is at least 1,000 times as much as the 50% inhibition activity against said immunoreaction by the N 1 -acetylspermidine in the sample. 
   
   
       2 . The monoclonal antibody according to  claim 1 , wherein the concentration of the diacetylspermine in the sample is 1 nM or less. 
   
   
       3 . A cell strain which produces the monoclonal antibody according to  claim 1 . 
   
   
       4 . A reagent for detecting diacetylspermine, comprising the monoclonal antibody according to  claim 1  or  2 .