US2010086984A1PendingUtilityA1
Optoinjection methods
Est. expiryMar 27, 2017(expired)· nominal 20-yr term from priority
C12N 13/00C12M 35/02C12M 47/06C12N 5/0087C12N 5/0093C12N 15/1034C12N 15/87G01N 33/5005G01N 33/5091G01N 33/56966
69
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Claims
Abstract
Optoinjection method for transiently permeabilizing a target cell by (a) illuminating a population of cells contained in a frame; (b) detecting at least one property of light directed from the frame; (c) locating a target cell by the property of light; and (d) irradiating the target cell with a pulse of radiation.
Claims
exact text as granted — not AI-modified1 . A method for transiently permeabilizing a target cell, comprising the steps of: (a) illuminating a population of substantially stationary cells contained in a frame; (b) obtaining a static representation of at least one property of light directed simultaneously from the frame; (c) locating a target cell in the population of cells, wherein the target cell is located with reference to the static representation; and (d) irradiating the target cell with a pulse of radiation, whereby the target cell is transiently permeabilized.
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