US2010083400A1PendingUtilityA1

Lolium Multiflorum Line Inducing Genome Loss

65
Individually held — no corporate assignee on recordPriority: Sep 30, 2008Filed: Sep 23, 2009Published: Apr 1, 2010
Est. expirySep 30, 2028(~2.2 yrs left)· nominal 20-yr term from priority
Inventors:Bryan K. Kindiger
A01H 1/021A01H 1/022A01H 5/12A01H 5/10A01H 6/4618C12N 5/04
65
PatentIndex Score
0
Cited by
0
References
0
Claims

Abstract

Dihaploid homozygous Fescue species may be produced by interspecific hybridization of Fescue with novel ryegrass, Lolium multiflorum , lines, that induce genome loss. Plants that are dihaploid homozygous Lolium and amphidiploids are also produced.

Claims

exact text as granted — not AI-modified
1 . A  Lolium multiflorum  diploid plant or parts thereof which segregates for levels of pollen sterility and where upon using the  Lolium  as the maternal parent in an interspecific hybridization with a  Festuca  species, being utilized as the paternal parent, confers to the resultant sterile F1 interspecific hybrids the ability to produce some seed via parthenogenesis that also possess a viable embryo, wherein said seeds which possess a viable embryo, may develop into F2 generation plants at least some of which are dihaploid homozygous  Festuca  species comprising a karyotype exhibiting a balanced genome of  Festuca  chromosomes equivalent to the chromosome number of said  Festuca  paternal parent and no Lolium chromosomes. 
   
   
       2 . The  Lolium multiflorum  diploid line of  claim 1  selected from the group consisting of IL-1, deposited as ATCC deposit accession no. PTA-10229, and progeny thereof. 
   
   
       3 . The  Lolium multiflorum  diploid line of  claim 1  selected from the group consisting of IL-2, deposited as ATCC deposit accession no. PTA-10315, and progeny thereof. 
   
   
       4 . The  Lolium multiflorum  diploid line of  claim 1  wherein said seed that possess a viable embryo develops into F2 generation plants at least some of which are dihaploid homozygous  Festuca  species comprising a karyotype of 42 Festuca chromosomes when said  Festuca  species paternal parent is Tall Fescue,  Festuca arundinaceae.    
   
   
       5 . Seed of said  Lolium multiflorum  diploid line of  claim 1 . 
   
   
       6 . An ovule of said  Lolium multiflorum  diploid line of  claim 1 . 
   
   
       7 . A tissue culture of regenerable cells of said  Lolium multiflorum  diploid line of  claim 1 . 
   
   
       8 . A method for producing fertile dihaploid homozygous  Festuca  species comprising:
 a) providing the  Lolium multiflorum  diploid line of  claim 1 ;   b) crossing said  Lolium multiflorum  diploid line as the maternal parent with a  Festuca  species utilized as the paternal parent to generate sterile F1 interspecific hybrid plants;   c) recovering and planting the panicle obtained from said F1 interspecific hybrid plants, wherein those of said seed heads that have developed embryos via parthenogenesis will develop into F2 plants; and   d) growing said F2 plants, wherein at least some of said F2 plants comprise dihaploid homozygous  Festuca  species comprising a karyotype with a balanced genome of  Festuca  chromosomes equivalent to the chromosome number of said  Festuca  paternal parent and no  Lolium  chromosomes.   
   
   
       9 . The method of  claim 8  wherein said F2 plants further comprise dihaploid homozygous  Lolium multiflorum  possessing a karyotype of 14  Lolium multiflorum  chromosomes and no Festuca chromosomes; amphidiploids comprising a karyotype of 14  Lolium multiflorum  chromosomes and  Festuca  chromosomes equivalent to the chromosome number of said  Festuca  male parent; or both. 
   
   
       10 . The method of  claim 9  further comprising recovering at least one of said F2 plants. 
   
   
       11 . The method of  claim 8  wherein said  Festuca  species male parent is Tall Fescue,  Festuca arundinaceae , and said dihaploid homozygous  Festuca  species F2 plants comprise a karyotype of 42  Festuca  chromosomes. 
   
   
       12 . The method of  claim 11  wherein said F2 plants further comprise dihaploid homozygous  Lolium  species comprising a karyotype of 14  Lolium multiflorum  chromosomes and no  Festuca  chromosomes, amphidiploids comprising a karyotype of 14  Lolium multiflorum  chromosomes and 42  Festuca  chromosomes, or both. 
   
   
       13 . The method of  claim 8  wherein said  Lolium multiflorum  diploid line is used as the maternal parent and is selected from the group consisting of IL-1 (ATCC deposit accession no. PTA-10229) and progeny thereof. 
   
   
       14 . The method of  claim 8  wherein said  Lolium multiflorum  diploid line is used as the maternal parent and is selected from the group consisting of IL-2 (ATCC deposit accession no. PTA-10315) and progeny thereof. 
   
   
       15 . The method of  claim 8  wherein said F2 plants comprise extranuclear genes of  Lolium multiflorum    
   
   
       16 . A dihaploid homozygous  Festuca  species produced by the process of  claim 8 , comprising extranuclear genes of  Lolium multiflorum.    
   
   
       17 . The dihaploid homozygous  Festuca  species produced by the process of  claim 11 , comprising extranuclear genes and cytoplasm of  Lolium multiflorum.    
   
   
       18 . A method for producing fertile dihaploid homozygous  Festuca  species comprising:
 a) providing the  Lolium multiflorum  diploid line of  claim 1 ;   b) crossing said  Lolium multiflorum  diploid line as a maternal parent with a  Festuca  species as the paternal parent to generate sterile F1 interspecific hybrid plants;   c) identifying somatic sectors in said F1 plants or in plants which are progeny thereof in which somatic sectors appear as fescue growing shoots comprising a dihaploid homozygous  Festuca  karyotype with all  Festuca  chromosomes and no  Lolium  chromosomes; and   d) retaining said F1 plants or said progeny in (c) which are identified as possessing said somatic sectors or recovering said somatic sectors therefrom;    wherein said somatic sectors develop into mature plants comprising dihaploid homozygous  Festuca  species comprising a karyotype exhibiting a balanced genome of  Festuca  chromosomes equivalent to the chromosome number of said  Festuca  patenal parent and no  Lolium  chromosomes.   
   
   
       19 . The method of  claim 18  wherein said progeny are produced by recovering and sowing seed from the panicles of said F1 hybrid plants and allowing said seed to develop into said progeny. 
   
   
       20 . The method of  claim 18  further comprising growing a mature dihaploid homozygous  Festuca  plant from either said plant or said progeny retained in (d) or from said somatic sector recovered in (d), wherein said adult dihaploid homozygous  Festuca  plant comprises a karyotype with a balanced genome of  Festuca  chromosomes equivalent to the chromosome number of said  Festuca  male parent and no  Lolium  chromosomes. 
   
   
       21 . The method of  claim 18  wherein said  Festuca  species male parent is Tall Fescue,  Festuca arundinaceae , and said mature plants comprise a karyotype of 42  Festuca  chromosomes 
   
   
       22 . The method of  claim 18  wherein said  Lolium multiflorum  diploid line used as the maternal parent is selected from the group consisting of IL-1 (ATCC deposit accession no. PTA-10229) and progeny thereof. 
   
   
       23 . The method of  claim 18  wherein said  Lolium multiflorum  diploid line used as the maternal parent is selected from the group consisting of IL-2 (ATCC deposit accession no. PTA-10315) and progeny thereof. 
   
   
       24 . The method of  claim 18  wherein said mature plants comprise extranuclear genes and cytoplasm of  Lolium multiflorum  genes. 
   
   
       25 . The method of  claim 18  further comprising:
 e) identifying somatic sectors in said F1 plants or in plants which are progeny thereof in which somatic sectors appear as ryegrass growing shoots comprising a dihaploid homozygous  Lolium  karyotype with all  Lolium  chromosomes and no  Festuca  chromosomes; and   f) retaining said F1 plants or said progeny in (e) which are identified as possessing said somatic sectors which appear as ryegrass, or recovering said somatic sectors which appear as ryegrass, therefrom;   
     wherein said somatic sectors which appear as ryegrass develop into mature plants comprising dihaploid homozygous  Lolium multiflorum  comprising a karyotype of 14  Lolium multiflorum  chromosomes and no  Festuca  chromosomes. 
   
   
       26 . A dihaploid homozygous  Festuca  species produced by the process of  claim 18 , comprising extranuclear genes of  Lolium multiflorum.    
   
   
       27 . The dihaploid homozygous  Festuca  species produced by the process of  claim 21 , comprising extranuclear genes of  Lolium multiflorum.    
   
   
       28 . The dihaploid homozygous  Lolium multiflorum  produced by the process of  claim 9 . 
   
   
       29 . The dihaploid homozygous  Lolium multiflorum  produced by the process of  claim 25 .

Join the waitlist — get patent alerts

Track US2010083400A1 — get alerts on status changes and closely related new filings.

We store only your email — no account needed. See our privacy policy.