Compositions and methods for stem cell delivery

Abstract

This invention provides compositions of matter, articles of manufacture and methods for delivering and/or affixing a stem cell to a target tissue. This invention also provides related nucleic acids, vectors, cell, methods of production, and kits.

1 - 91 . (canceled) 
   
   
       92 . A composition of matter for delivering and/or affixing a stem cell to a target tissue comprising a first binding moiety that specifically binds to a first antigen on the surface of the stem cell, fused to a second binding moiety that specifically binds to a second antigen on the surface of a cell in the target tissue. 
   
   
       93 . The composition of  claim 92 , wherein the first antigen is selected from the group consisting of CD34, CD45, IL-3R, IL-6R, IL-11R, VLA-4, VLA-5, L-selectin, PECAM-1, beta-1 integrin and Sca-1. 
   
   
       94 . The composition of  claim 92 , wherein the second antigen is selected from the group consisting of myosin, cardiac troponin T, cardiac troponin I, actin, beta-myosin heavy chain, tropomyosin, ICAM-1, P-selectin, IL-8, and P63. 
   
   
       95 . The composition of  claim 93 , wherein the second antigen is VCAM-1. 
   
   
       96 . The composition of  claim 93 , wherein the second antigen is ICAM-1. 
   
   
       97 . The composition of  claim 93 , wherein the second antigen is myosin. 
   
   
       98 . The composition of  claim 94 , wherein the first antigen is CD34. 
   
   
       99 . The composition of  claim 94 , wherein the first antigen is CD45. 
   
   
       100 . The composition of  claim 94 , wherein the first antigen is c-kit. 
   
   
       101 . The composition of  claim 94 , wherein the first antigen is Sca-1. 
   
   
       102 . The composition of  claim 92 , wherein the first antigen is CD34 and the second antigen is selected from the group consisting of VCAM-1, myosin, and ICAM-1. 
   
   
       103 . The composition of  claim 92 , wherein the first antigen is CD45 and the second antigen is selected from the group consisting of VCAM-1, myosin, and ICAM-1. 
   
   
       104 . The composition of  claim 92 , wherein the first antigen is c-kit and the second binding moiety is myosin, or ICAM-1. 
   
   
       105 . The composition of  claim 92 , wherein the first antigen is Sca-1 and the second antigen is selected from the group consisting of VCAM-1, myosin, and ICAM-1. 
   
   
       106 . The composition of  claim 92 , wherein the composition comprises a bi-specific antibody. 
   
   
       107 . A method for delivering and/or affixing a stem cell to a target tissue, the method comprising contacting the target tissue with the stem cell and a composition comprising a first binding moiety that specifically binds to a first antigen on the surface of the stem cell, fused to a second binding moiety that specifically binds to a second antigen on the surface of a cell in the target tissue. 
   
   
       108 . The method of  claim 107 , wherein the stem cell is first contacted with the composition to permit formation of a complex between the first binding moiety and the stem cell, and the resulting complex is then contacted with the target tissue. 
   
   
       109 . The method of  claim 107 , wherein the target tissue is first contacted with the composition to permit formation of a complex between the second binding moiety and the target tissue, the stem cell is then contacted with the resulting complex. 
   
   
       110 . The method of  claim 107 , wherein the composition comprises a bi-specific antibody. 
   
   
       111 . A kit comprising the composition of  claim 92 , and instructions for use.