US2010041753A1PendingUtilityA1

Inhibition of biofilm formation

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Assignee: SEQUOIA SCIENCE INCPriority: Jul 14, 2004Filed: Oct 8, 2009Published: Feb 18, 2010
Est. expiryJul 14, 2024(expired)· nominal 20-yr term from priority
Inventors:Gary Eldridge
A61P 31/04C07K 14/245C07K 14/21C07K 14/28A61K 31/203C12Q 1/025C12Q 1/6897Y02A50/30
60
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Claims

Abstract

The present invention relates to compositions and methods for reducing or inhibiting biofilm comprising modulating expression of a cysB gene in a cell. The invention also provides methods for modulating the expression of a cysB, cysD, cysI, cysJ, cysK, and ybiK. The invention further provides methods for identifying gene(s) involved in biofilm formation and for identifying biofilm inhibitors.

Claims

exact text as granted — not AI-modified
1 . A method for reducing or inhibiting a biofilm comprising modulating expression of a gene present in a Gram-negative bacteria cell capable of biofilm formation, the gene being a cysD, cysI, cysJ, cysK or ybiK gene, thereby to reduce or inhibit the biofilm formation. 
   
   
       2 . The method of  claim 1 , wherein modulation of the gene comprises contacting the cell with a composition comprising a compound selected from the group consisting of ursolic acid and asiatic acid, or a pharmaceutically acceptable salt of such compound, a hydrate of such compound, a solvate of such compound, an N-oxide of such compound, or a combination thereof. 
   
   
       3 . The method of  claim 2 , wherein the compound is ursolic acid. 
   
   
       4 . The method of  claim 2 , wherein the compound is asiatic acid. 
   
   
       5 . The method of  claim 1 , wherein the cell is selected from a group consisting of  Escherichia coli, Proteus mirablis, Francisella tularensis, Vibrio  sp.,  Pseudomonas aeruginosa, V. harveyi, Pseudomonas  sp.,  Salmonella  sp.,  Haemophilus influenzae, Borrelia  sp.,  Neisseria  sp.,  Bacillus  sp.,  Burkholderia  sp.,  Klebsiella  sp., and  Yersinia pestis.    
   
   
       6 . The method of  claim 5 , wherein the cell is  Escherichia coli.    
   
   
       7 . The method of  claim 5 , wherein the cell is  Pseudomonas aeruginosa.    
   
   
       8 . The method of  claim 5 , wherein the cell is  V. harveyi.    
   
   
       9 . (canceled) 
   
   
       10 . The method of  claim 2 , wherein the gene is cysD. 
   
   
       11 . The method of  claim 2 , wherein the gene is cysI. 
   
   
       12 . The method of  claim 2 , wherein the gene is cysJ. 
   
   
       13 . The method of  claim 2 , wherein the gene is cysK. 
   
   
       14 . The method of  claim 2 , wherein the gene is ybiK. 
   
   
       15 . A method for modulating the expression of a gene selected from the group consisting of cysD, cysI, cysK or ybiK, comprising contacting a Gram-negative bacteria cell capable of biofilm formation with a composition comprising a compound selected from the group consisting of ursolic acid and asiatic acid, or a pharmaceutically acceptable salt of such compound, a hydrate of such compound, a solvate of such compound, a N-oxide of such compound, or a combination thereof. 
   
   
       16 . The method of  claim 15 , wherein the compound is ursolic acid. 
   
   
       17 . The method of  claim 15 , wherein the compound is asiatic acid. 
   
   
       18 . The method of  claim 15 , wherein the gene is cysD. 
   
   
       19 . The method of  claim 15 , wherein the gene is cysI. 
   
   
       20 . The method of  claim 15 , wherein gene is cysJ. 
   
   
       21 . The method of  claim 15 , wherein the gene is cysK. 
   
   
       22 . The method of  claim 15 , wherein the gene is ybiK. 
   
   
       23 . The method of  claim 15 , wherein the cell is selected from a group consisting of  Escherichia coli, Proteus mirablis, Francisella tularensis, Vibrio  sp.,  Pseudomonas aeruginosa, V. harveyi, Pseudomonas  sp.,  Salmonella  sp.,  Haemophilus influenzae, Borrelia  sp.,  Neisseria  sp.,  Bacillus  sp.,  Burkholderia  sp.,  Klebsiella  sp., and  Yersinia pestis.    
   
   
       24 . The method of  claim 23 , wherein the cell is  Escherichia coli.    
   
   
       25 . The method of  claim 23 , wherein the cell is  Pseudomonas aeruginosa.    
   
   
       26 . The method of  claim 23 , wherein the cell is  V. harveyi.    
   
   
       27 . The method of  claim 15 , wherein the cell is selected from a group consisting of Gram-negative bacteria. 
   
   
       28 .- 38 . (canceled)

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