Process for Producing Anticancer Agent LL-D45042

Abstract

The disclosure describes the production of anticancer agent LL-D45042, having the structure: by fermentation, to methods for the recovery and concentration of this anticancer agent from crude solutions, and to processes for the purification of this anticancer agent as well as a new microorganism of the species Streptomyces hygroscopicus LL-D45042 and mutants thereof useful in the preparation of this compound.

1 . A process for the preparation of LL-D45042 which comprises cultivating  Streptomyces hygroscopicus  LL-D45042 or a mutant thereof under aerobic conditions, in a sterile liquid medium containing assimilable sources of carbon, nitrogen and inorganic anion and cation salts, until LL-D45042 is formed and recovering and isolating LL-D45042. 
   
   
       2 . A biologically pure culture  Streptomyces hygroscopicus  LL-D45042 or a LL-D45042 producing mutant thereof. 
   
   
       3 . A biologically pure culture of the microorganism  Streptomyces hygroscopicus , LL-D45042 or a mutant thereof, having the ability to produce a compound having the structure 
     
       
         
         
             
             
         
       
     
     on aerobic fermentation in a nutrient medium. 
   
   
       4 . A method of treating a mammal affected by cancer, which method comprises administering to said mammal an effective amount of a compound having the structure. 
     
       
         
         
             
             
         
       
     
   
   
       5 . (canceled) 
   
   
       6 . An anticancer agent LL-D45042 produced by the process which comprises aerobically cultivating the organism  Streptomyces hygroscopicus  LL-D45042 or a mutant thereof in a sterile liquid medium containing assimilable sources of carbon, nitrogen and inorganic anion and cation salts until LL-D45042 is formed and recovering and isolating LL-D45042.