Cellomics systems

Abstract

In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

1 . A biological sample analysis chip with a different probe fixed on each area of a plurality of areas discretely provided on a substrate thereof, each of the plurality of discrete areas having an area not more than that of a circle 700 nmφ and not less than that of a circle 3 nmφ. 
     
     
         2 . A biological sample analysis chip with a different probe fixed on each area of a plurality of areas discretely provided on a substrate thereof, providing structures each having a specific shape on the four corners in each of the plurality of discrete areas, and each of the structures having a specific shape provided on the four corners being different from one another in each of the plurality of discrete areas respectively. 
     
     
         3 . The biological sample analysis chip according to  claim 1 , wherein the areas having as a unit a prespecified number of the plurality of discrete areas are each provided via a groove formed between the areas. 
     
     
         4 . The biological sample analysis chip according to  claim 1 , wherein the areas having as a unit a prespecified number of the plurality of discrete areas are provided at a prespecified distance or farther. 
     
     
         5 . An analysis method by means of a biological sample analysis chip comprising the steps of:
 dropping a sample solution on a biological sample analysis chip with a different probe fixed on each of a plurality of areas discretely provided on a substrate thereof, each of the plurality of discrete areas having an area not more than that of a circle 700 nmφ and not less than that of a circle 3 nmφ;   placing a thin plate or a rod rotating at a prespecified speed on the top face of the biological sample analysis chip; and   moving the plate or rod from side to side on the substrate to accelerate hybridization between the probe and a sample in the sample solution.   
     
     
         6 . An analysis method by means of a biological sample analysis chip comprising the steps of:
 dropping a sample solution on a biological sample analysis chip with a different probe fixed on each area of a plurality of areas discretely provided on a substrate thereof, a structure having a specific shape being provided on each of the four corners in the plurality of discrete areas, and each of the structures having a specific shape provided on the four corners being different from one another with respect to each of the plurality of discrete areas;   placing a thin plate or a rod rotating at a prespecified speed on the top face of the biological sample analysis chip; and   moving the plate or rod from side to side on the substrate to accelerate hybridization between the probe and a sample in the sample solution.   
     
     
         7 . A method of analyzing a biological sample comprising the steps of:
 tracing with a probe for an atomic force microscope a biological sample analysis chip with a different probe fixed on each area of a plurality of areas discretely provided on a substrate thereof, each of the plurality of discrete areas having an area not more than that of a circle 700 nmφ and not less than that of a circle 3 nmφ; and   detecting a sample hybridized with the probe.   
     
     
         8 . A method of analyzing a biological sample comprising the steps of:
 tracing with a probe for an atomic force microscope a biological sample analysis chip with a different probe fixed on each area of a plurality of areas discretely provided on a substrate thereof, a structure having a specific shape being provided on each of the four corners in the plurality of discrete areas, and each of the structures having a specific shape provided on the four corners being different from one another with respect to each of the plurality of discrete areas; and   detecting a sample hybridized with the probe.   
     
     
         9 . The method of analyzing a biological sample according to  claim 7 , wherein the sample hybridized with the probe reacts to a labeling probe labeling particles each having a different particle diameter to an oligo probe hybridized with a sequence portion complementary to and different from a DNA fragment having been hybridized.