Cellomics system

Abstract

In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

1 . A method of collecting an electrophoretic separated substance comprising the steps of:
 irradiating convergence light to a specific electrophoretic separation band portion of the electrophoretic separation band developed on heat-melting gel; and   melting the electrophoretic separation band portion to collect the same.   
     
     
         2 . A device for collecting an electrophoretic separated substance comprising: a means for holding an electrophoretic separation gel substrate having a electrophoretic separation band developed on heat-melting gel; a means for detecting a specific electrophoretic separation band portion of the electrophoretic separation band; a means for irradiating convergence light to the detected electrophoretic separation band portion to heat the same; and a means for sucking gel melted by the heating. 
     
     
         3 . The device for collecting an electrophoretic separated substance according to  claim 2 , wherein the means for holding an electrophoretic separation gel substrate holds a means for regulating temperature. 
     
     
         4 . The device for collecting an electrophoretic separated substance according to  claim 2 , wherein the means for sucking gel melted by the heating is provided with a means for accessing a pipet and a specific electrophoretic separation band portion with the pipet melted thereon. 
     
     
         5 . The device for collecting an electrophoretic separated substance according to  claim 2 , wherein the heat-melting gel contains agarose in a quantity at least sufficient to maintain a gel structure thereof. 
     
     
         6 . The device for collecting an electrophoretic separated substance according to  claim 2 , wherein the melting point of agarose for the heat-melting gel is 60° C. or below. 
     
     
         7 . A heat-melting gel substrate applied to a method of collecting an electrophoretic separated substance comprising the steps of: irradiating convergence light to a specific electrophoretic separation band portion of the electrophoretic separation band developed on heat-melting gel; and melting the electrophoretic separation band portion to collect the same, the heat-melting gel being fixed onto a glass substrate with a thickness of the heat-melting gel not less than 0.02 mm nor more than 0.2 mm. 
     
     
         8 . A device for collecting an electrophoretic separated substance configured to have the means for sucking gel melted by heating capable of impressing electric field with a pipet with a first electrode attached to the inside thereof between the first electrode and another electrode provided in a vessel outside. 
     
     
         9 . A method of collecting an electrophoretic separated substance comprising the steps of:
 filling the pipet with an electrolysis solution in advance;   sucking gel melted by convergence light;   lowering the temperature of the gel sucked in the pipet to turn the same into gel again;   contacting a pipet chip tip section with a vessel filled with an electrolysis solution;   impressing electric field between a first electrode contacting the electrolysis solution in the pipet and a second electrode in the vessel, taking the second electrode as positive pole, to subject an electrophoretic separated substance contained in the gel solidifying in the pipet chip to electrophoresis to elute the same in the electrolysis solution in the vessel.