Arabidopsis derived promoters for regulation of plant expression

Abstract

The invention provides a method to identify a plurality of plant promoters having specified characteristics and promoters identified by the method. Also provided are transgenic plants comprising the genes identified by the methods of the invention.

1 . An isolated polynucleotide comprising a wound inducible promoter that directs transcription of an operably linked heterologous nucleic acid sequence in a plant cell, wherein the wound inducible promoter comprises SEQ ID NO: 17. 
     
     
         2 . An expression cassette comprising the polynucleotide of  claim 1 , wherein the heterologous nucleic acid sequence is an open reading frame. 
     
     
         3 . The expression cassette of  claim 2 , wherein the open reading frame comprises a coding sequence which, when transcribed at the direction of the polynucleotide, imparts a phenotype selected from the group consisting of insect resistance, disease resistance, herbicide resistance, abiotic stress resistance, a modified enzyme expression profile, a modified oil content, and a modified nutrient content. 
     
     
         4 . A transformed plant, the genome of which comprises the expression cassette of  claim 3 . 
     
     
         5 . A cell of the transformed plant of  claim 5 . 
     
     
         6 . The transformed plant of  claim 6 , wherein the plant is a monocot or a dicot plant. 
     
     
         7 . The transformed monocot plant of  claim 7  wherein the plant is a cereal plant. 
     
     
         8 . The cereal plant of  claim 8  wherein the plant is selected from the group consisting of maize, wheat, rice, sorghum, and barley. 
     
     
         9 . The transformed dicot plant of  claim 7  wherein the dicot is selected from the group consisting of soybean, cotton, canola, and sugarbeet. 
     
     
         10 . An isolated polynucleotide sequence, wherein the polynucleotide sequence is SEQ ID NO: 17.