Cellomics system

Abstract

In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

1 . A DNA probe chip comprising:
 a substrate;   an electrode formed on the substrate and having a plurality of discrete probe fixing areas formed thereon; and   prespecified DNA probes each fixed on respective probe fixing areas with covalent bonding,   wherein the DNA probe being configured to cause hybridization with a complementary strand DNA from the terminus side fixed onto the probe fixing area.   
     
     
         2 . A DNA probe chip comprising:
 a substrate;   an electrode formed on the substrate and having a plurality of discrete probe fixing areas formed thereon; and   prespecified DNA probes each fixed on respective probe fixing areas with covalent bonding,   wherein a dissociative group having a negative charge on the terminus different from that fixed on the probe fixing area being added to each of the DNA probes.   
     
     
         3 . The DNA probe chip according to  claim 1 , wherein the electrode with a plurality of discrete probes formed thereon is an ITO electrode, and a water repellent surface coating is applied to the area other than the probe fixing areas. 
     
     
         4 . The DNA probe chip according to  claim 1 , wherein an electrode surface in the plurality of discrete probe fixing areas is prepared by introducing a residue dissociating positive. 
     
     
         5 . The DNA probe chip according to  claim 1 , wherein the DNA probe is a PNA whose main chain is composed with the peptide bond, or a CAS having S-carboxymethyl-L-cysteine as a basic skeleton. 
     
     
         6 . The DNA probe chip according to  claim 1 , wherein the DNA probe is designed to have a sequence complementary to that having a prespecified base length starting from an mRNA sequence having a sequence to be hybridized with the DNA probe, and the DNA probe is selected from an area in which the quantity of GC fixed onto the plurality of discrete probe fixing areas on the terminus side is higher than that on the free end side. 
     
     
         7 . The DNA probe chip according to  claim 1 , wherein the DNA probe has a sequence complementary to that having a prespecified base length starting from an mRNA sequence having a sequence to be hybridized with the DNA probe, and the DNA probe has a configuration in which, between the terminus side fixed onto the plurality of discrete probe fixing areas and the free end side is inserted a sequence mismatched with a sequence to be hybridized with the DNA probe between the position about 10 bases and that about 30 bases from the free end, or a blank sequence not forming a stable complementary strand with any of ACGT. 
     
     
         8 . A method of controlling DNA hybridization comprising the steps of:
 adding a sample solution containing a target polynucleotide to between a DNA probe chip comprising: a substrate; an electrode with a plurality of discrete probe fixing areas formed on the substrate formed thereon; and prespecified DNA probes each fixed on respective probe fixing areas with covalent bonding, a dissociative group having a negative charge on the terminus different from that fixed on the probe fixing area being added to the DNA probe: and a member provided opposing to a surface of the DNA probe chip;   applying prespecified electric field to between the electrode and the sample solution site to condense the polynucleotide in the proximity of the surface of the DNA probe chip; and   inverting the electric field for applying to between the electrode and the sample solution site to start hybridization in the state where the probe is stretched.   
     
     
         9 . The method of controlling DNA hybridization according to  claim 7 , wherein a DNA probe chip is employed in which the electrode formed thereon a plurality of discrete probe is an ITO electrode, and a water repellent surface coating is applied to the area other than the probe fixing areas. 
     
     
         10 . The method of controlling DNA hybridization according to  claim 7 , wherein a DNA probe chip is employed in which an electrode surface in the plurality of discrete probe fixing areas is prepared by introducing a residue dissociating positive. 
     
     
         11 . The method of controlling DNA hybridization according to  claim 7 , wherein a DNA probe chip is employed in which the DNA probe is a PNA whose main chain is composed with the peptide bond, or a CAS having S-carboxymethyl-L-cysteine as a basic skeleton. 
     
     
         12 . A DNA probe chip comprising:
 a substrate;   an electrode with a plurality of discrete probe fixing areas formed on the substrate formed thereon; and   prespecified DNA probes each fixed on respective probe fixing areas with covalent bonding,   wherein the DNA probe being configured to form more stable hybridization on the terminus side fixed onto the probe fixing area than on the free end side.   
     
     
         13 . The DNA probe chip according to  claim 1 , wherein the DNA probe with one end thereof fixed on a substrate has a sequence complementary to that having a prespecified base length starting from an mRNA sequence having a sequence to be hybridized with the DNA probe, and the DNA probe is selected from an area in which the quantity of GC fixed onto the plurality of discrete probe fixing areas on the terminus side is higher than that on the free end side.