Polypeptide having larvae growth inhibiting or insecticidal effect on scarabaeidae insects and polynucleotide encoding the same

Abstract

Through screening using an antibody to a polypeptide that constitutes a parasporal body in sporangia of Bacillus popilliae and has a larvae growth inhibiting or insecticidal effect on a Scarabaeidae insect and through polymerase chain reaction, polynucleotides encoding the polypeptide and polypeptides similar thereto are isolated.

1 - 27 . (canceled) 
     
     
         28 . A method for controlling a Scarabaeidae insect, which comprises a step of administering orally a Scarabaeidae insect controlling agent comprising as an active ingredient a polypeptide having the amino acid sequence of SEQ ID NO: 20 to the Scarabaeidae insect. 
     
     
         29 . The method according to  claim 28 , wherein the Scarabaeidae insect controlling agent further comprises a component selected from the group consisting of excipients, carriers, nutritive agents and stabilizers. 
     
     
         30 . The method according to  claim 28 , wherein the Scarabaeidae insect controlling agent further comprises sporangia of a bacterium belonging to  Bacillus popilliae.    
     
     
         31 . The method according to  claim 29 , wherein the Scarabaeidae insect controlling agent further comprises sporangia of a bacterium belonging to  Bacillus popilliae.    
     
     
         32 . The method according to  claim 29 , wherein the Scarabaeidae insect controlling agent further comprises a component selected from the group consisting of mineral fine powders selected from the group consisting of kaolin clay, bentonite, montmorillonite, diatomaceous earth, acid clay, talcs, pearlite, vermiculite, ammonium sulfate, urea, ammonium chloride, ammonium nitrate, wheat bran, chitin, polysaccharides, rice bran, and flour, as solid carriers; fixing agents and dispersants selected from the group consisting of casein, gelatin, gum Arabic, alginic acid, saccharides, synthetic polymers, polyvinyl alcohol, and polyacrylic acids; and cryoprotectants selected from the group consisting of propylene glycol, ethylene glycol, xanthan gum and polyacrylic acids. 
     
     
         33 . The method according to  claim 29 , wherein a ratio of polypeptide to other components is 0.0001 to 100% by mass. 
     
     
         34 . The method according to  claim 28 , wherein the Scarabaeidae insect is selected from the group consisting of  Anomala cuprea, Blitopertha orientalis, Popilliae japonica, Phyllopertha diversa, Adoretus tenuimaculatus  and  Anomala rufocuprea.    
     
     
         35 . The method according to  claim 28 , wherein an application amount of polypeptide is 0.01 to 100 g/m 2  for ground dispersion.