Isolated proteins MAGE-4 and MAGE-41

Abstract

The invention relates to nucleic acid molecules which code for the tumor rejection antigen precursor MAGE-3. Also disclosed are vectors, cell lines, and so forth, which utilize the nucleic acid molecule, and optionally, molecules coding for human leukocyte antigen HLA-A1. Uses of these materials in therapeutic and diagnostic contexts are also a part of the invention.

1 - 21 . (canceled) 
     
     
         22 . An isolated nucleic acid molecule selected from the group consisting of:
 (i) the nucleotide sequence set forth in SEQ ID NO: 13, and   (ii) the nucleotide sequence set forth in SEQ ID NO: 14.   
     
     
         23 . An isolated nucleic acid molecule selected from the group consisting of:
 (i) nucleotides 625-1578 of SEQ ID NO: 13, and   (ii) nucleotides 625-1578 of SEQ ID NO: 14.   
     
     
         24 . An expression vector comprising the isolated nucleic acid molecule of  claim 22 , operably linked to a promoter. 
     
     
         25 . An expression vector comprising the isolated nucleic acid molecule of  claim 23 , operably linked to a promoter. 
     
     
         26 . Cell line transformed or transfected with the expression vector of  claim 24 . 
     
     
         27 . Cell line transformed or transfected with the expression vector of  claim 25 . 
     
     
         28 . The isolated nucleic acid molecule of  claim 22 , consisting of the nucleotide sequence of SEQ ID NO: 13. 
     
     
         29 . The isolated nucleic acid molecule of  claim 22 , consisting of the nucleotide sequences of SEQ ID NO: 14. 
     
     
         30 . The isolated nucleic acid molecule of  claim 23 , consisting of nucleotides 625-1578 of SEQ ID NO: 13. 
     
     
         31 . The isolated nucleic acid molecule of  claim 24 , consisting of nucleotides 625-1578 of SEQ ID NO: 14. 
     
     
         32 . An expression vector comprising the isolated nucleic acid molecule of  claim 28 , operably linked to a promoter. 
     
     
         33 . An expression vector comprising the isolated nucleic acid molecule of  claim 29 , operably linked to a promoter. 
     
     
         34 . An expression vector comprising the isolated nucleic acid molecule of  claim 30 , operably linked to a promoter. 
     
     
         35 . An expression vector comprising the isolated nucleic acid molecule of  claim 31 , operably linked to a promoter. 
     
     
         36 . Cell line transformed or transfected with the expression vector of  claim 32 . 
     
     
         37 . Cell line transformed or transfected with the expression vector of  claim 33 . 
     
     
         38 . Cell line transformed or transfected with the expression vector of  claim 34 . 
     
     
         39 . Cell line transformed or transfected with the expression vector of  claim 35 . 
     
     
         40 . An expression vector comprising the isolated nucleic acid molecule of  claim 22 , and at least one other isolated nucleic acid molecule which encodes a protein. 
     
     
         41 . An expression vector comprising the isolated nucleic acid molecule of  claim 23 , and at least one other isolated nucleic acid molecule which encodes a protein. 
     
     
         42 . An isolated protein encoded by a nucleic acid molecule consisting of a nucleotide sequence which hybridizes to the nucleotide sequence of SEQ ID NO: 11 or SEQ ID NO: 12, at the following conditions: four washes at 65° C., one hour each, in 2×SSC, 0.4% SDS, and 30 minutes at 0.01×SSC, 0.1% SDS 
     
     
         43 . The isolated protein of  claim 42 , the amino acid sequence of which includes an amino acid sequence of a MAGE-3 tumor rejection antigen.