US2010003746A1PendingUtilityA1
Production of Lentiviral Vectors
Individually held — no corporate assignee on recordPriority: Feb 12, 2007Filed: Feb 11, 2008Published: Jan 7, 2010
Est. expiryFeb 12, 2027(~0.5 yrs left)· nominal 20-yr term from priority
C12N 15/86C12N 2710/14043A61K 48/0091C12N 2740/15051C12N 2710/14021C12N 7/00C12N 2710/14051C12N 2740/15043C07K 14/005C12N 2740/16051C12N 2740/16022C12N 2740/16045C12N 2810/6081A61K 48/00C12N 2740/16043C12N 15/64C12N 15/79
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Claims
Abstract
The present invention is a method of generating a lentivirus vector, comprising cloning each of a leotivimus transfer construct, gag, pol, an envelope protein and rev respectively into the same or different baculoviruses, and transducing a producer cell with the or each baculovirus.
Claims
exact text as granted — not AI-modified1 . A method of generating a lentivirus vector, comprising cloning each of a lentivirus transfer construct, gag, pot an envelope protein and rev respectively into the same or different baculoviruses, and transducing a producer cell with the or each baculovirus.
2 . The method according to claim 1 , wherein the envelope protein is VSV-G.
3 . The method according to claim 2 , wherein the lentivirus transfer construct, gag, pol, VSV-G and rev are respectively cloned into BAC-transfer, BAC-gag-pol, BAC-VSVg and BAC-rev.
4 . The method according to claim 1 , wherein the lentivirus transfer construct, gag, pol, the envelope protein and rev are each cloned into the same baculovirus.
5 . The method, according to claim 1 , wherein the producer cell is 293T, HepG2, CHO, BHK, Sf9, Sf21, 293,BTI-Tn 5 B 1-4, COS, NIH/3T3, Vero, NSO or PerC6 cells.
6 . The method according to claim 1 , wherein the lentivirus transfer construct is a third-generation lentivirus transfer construct.Join the waitlist — get patent alerts
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