Live attenuated aldolase-negative bacterial vaccine

Abstract

A live attenuated bacterium of the genus Escherichia, Salmonella or Yersinia, said bacterium being incapable of expressing a functional Eda protein as a result of a mutation in the eda gene and a vaccine comprising the live attenuated bacterium.

1 . A live attenuated bacterium of the genus  Escherichia, Salmonella  or  Yersinia  having a mutation in the eda gene, said bacterium not being capable of expressing a functional Eda protein as a result of the mutation in the eda gene. 
     
     
         2 . The live attenuated bacterium according to  claim 1 , wherein said bacterium is selected from the group consisting of  E. coli, Y. pestis, S. enterica  serotype  typhimurium, enteritidis, choleraesuis, dublin, typhi, gallinarum, abortusovi, abortus - equi,  and  pullorum.    
     
     
         3 . The live attenuated bacterium according to  claim 1 , wherein the mutation comprises an insertion and/or a deletion. 
     
     
         4 . The live attenuated bacterium according to  claim 3 , wherein said bacterium carries a heterologous gene. 
     
     
         5 . The live attenuated bacterium according to  claim 4 , wherein said heterologous gene is inserted in the eda gene. 
     
     
         6 . A vaccine for the protection of animals or humans against infection with a pathogenic bacterium or the pathogenic effects thereof, wherein said vaccine comprises the live attenuated bacterium according to  claim 1  and a pharmaceutically acceptable carrier. 
     
     
         7 . The vaccine according to  claim 6 , comprising an adjuvant. 
     
     
         8 . The vaccine according to  claim 6 , in a freeze-dried form. 
     
     
         9 - 10 . (canceled)