Sugar Accumulation

Abstract

Methods and materials for modulating lysine, glucose, fructose, galactose and leucine content in plants are disclosed.

1 . A plant comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 and SEQ ID NO:13, wherein said plant has a difference in the level of at least one of lysine, glucose, fructose, and galactose compared to the corresponding level in a control plant that does not comprise said exogenous nucleic acid. 
     
     
         2 . The plant of  claim 1  wherein said amino acid sequence is SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:10. 
     
     
         3 . A plant comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 80% or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, and SEQ ID NO:28, wherein said plant has a difference in the level of leucine compared to the level of leucine in a control plant that does not comprise said exogenous nucleic acid. 
     
     
         4 . The plant of  claim 3 , wherein said amino acid is SEQ ID NO:15. 
     
     
         5 . A plant comprising an exogenous nucleic acid, wherein said exogenous nucleic acid comprises a regulatory region operably linked to a nucleotide sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 and SEQ ID NO:13; and a second exogenous nucleic acid comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, and SEQ ID NO:28, wherein said plant has a difference in the level of at least one of lysine, glucose, fructose, galactose and leucine as compared to the corresponding level in a control plant that does not comprise said first and said second exogenous nucleic acids. 
     
     
         6 . The plant of  claim 1 ,  3 , or  5 , wherein said sequence identity is greater than 85%. 
     
     
         7 . The plant of  claim 6 , wherein said sequence identity is greater than 90%. 
     
     
         8 . The plant of  claim 7 , wherein said sequence identity is greater than 95%. 
     
     
         9 . The plant of  claim 1 ,  3 , or  5 , wherein said difference is an increase. 
     
     
         10 . The plant of  claim 1 ,  3 , or  5 , wherein said difference is a decrease. 
     
     
         11 . The plant of  claim 1 ,  3 , or  5 , wherein said regulatory region is a promoter. 
     
     
         12 . The plant of  claim 11 , wherein said promoter is a cell-specific or tissue-specific promoter. 
     
     
         13 . The plant of  claim 12 , wherein said promoter is a broadly expressing promoter. 
     
     
         14 . The plant of  claim 13 , wherein said broadly expressing promoter is selected from the group consisting of the p326, YP0158, YP0214, YP0380, PT0848, PT0633, YP0050, YP0144 and YP0190 promoters. 
     
     
         15 . The plant of  claim 12 , wherein said tissue-specific promoter is a seed-specific promoter. 
     
     
         16 . The plant of  claim 15 , wherein said seed-specific promoter is selected from the group consisting of the napin promoter, the Arcelin-5 promoter, the phaseolin gene promoter, the soybean trypsin inhibitor promoter, the ACP promoter, the stearoyl-ACP desaturase gene promoter, the soybean α′ subunit of β-conglycinin promoter, the oleosin promoter, the 15 kD zein promoter, the 16 kD zein promoter, the 19 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the Osgt-1 promoter, the beta-amylase gene promoter, and the barley hordein gene promoter. 
     
     
         17 . The plant of  claim 12 , wherein said promoter is a vegetative tissue specific promoter. 
     
     
         18 . The plant of  claim 12 , wherein said promoter is a fruit specific promoter. 
     
     
         19 . The plant of  claim 11 , wherein said promoter is an inducible promoter. 
     
     
         20 . The plant of  claim 1 ,  3 , or  5 , wherein said plant is from a genus selected from the group consisting of  Abies, Agrostis, Allium, Alseodaphne, Anacardium, Andropogon, Arachis, Apium, Aragrostis, Ascophyllum, Asparagus, Atropa, Avena, Beilschmiedia, Brassica, Capsicum, Carthamus, Chondrus, Chicorium, Citrus, Citrullus, Cocculus, Cocos, Coffea, Corylus, Cracilaria, Croton, Crypthecodinium, Cucumis, Cucurbita, Cunninghamia, Cuphea, Cynodon, Daucus, Dianthus, Duguetia, Elaeis, Enteromorpha, Euphoria, Festuca, Festulolium, Ficus, Fragaria, Fucus, Glaucium, Glycine, Gossypium, Haematococcus, Helianthus, Heterocallis, Hevea, Himanthalia, Hordeum, Hyoscyamus, Lactuca, Landolphia, Lemna, Linum, Litsea, Lolium, Lycopersicon, Lupinus, Majorana, Malus, Manihot, Medicago, Musa, Nicotiana, Odontella, Olea, Oryza, Palmaria, Panicum, Pannesetum, Papaver, Parthenium, Persea, Petunia, Phaseolus, Phleum, Phoenix, Picea, Pinus, Pistacia, Pisum, Poa, Populus sect., Porphyra, Prunus, Pseudotsuga Pyrus, Raphanus, Ricinus, Rosa, Rubus, Saccharum, Salix, Schizochytrium, Secale, Senecio, Sinapis, Solanum, Sorghum, Spinacia, Spirulina, Stephania, Triticum, Tagetes, Theobroma, Trifolium, Trigonella, Ulva, Undaria, Vaccinium, Vicia, Vigna, Vinca, Vitis , and  Zea.    
     
     
         21 . The plant of  claim 1 ,  3 , or  5 , wherein said plant is a species selected from  Ananus comosus, Arabidopsis thaliana, Brassica rapa, Brassica napus, Brassica oleracea, Bixa orellana, Calendula officinalis, Cinnamommum camphora, Coffea arabica, Glycine max, Glycyrrhiza glabra, Gossypium hirsutum, Gossypium herbaceum, Lactuca sativa, Lycopersicon esculentum, Mentha piperita, Mentha spicata, Musa paradisiaca, Oryza sativa, Parthenium argentatum, Rosmarinus officinalis, Solanum tuberosum, Theobroma cacao, Triticum aestivum, Vitis vinifera , and  Zea mays.    
     
     
         22 . The plant of  claim 1 ,  3 , or  5 , wherein said plant is selected from the group consisting of alfalfa, amaranth, apple, kidney beans, lima beans, dry beans, green beans, broccoli, cabbage, carrot, castor bean, chick peas, cherry, clover, coffee, cotton, cottonseed, crambe, eucalyptus, flax, grape, grapefruit, lemon, lentils, lettuce, linseed, mango, watermelon, cantaloupe, mustard, orange, peanut, peach, pear, peas, pepper, plum, poplar, potato, high erucic acid rapeseed, canola, safflower, sesame, soybean, spinach, strawberry, sugarbeet, sunflower, tea, tomato, banana, barley, date palm, field corn, garlic, millet, oat, oil palm, onion, pineapple, popcorn, rice, rye, sorghum, sudangrass, sugarcane, sweet corn, switchgrass, turf grasses, wheat, fir, pine, spruce, brown seaweeds, green seaweeds, red seaweeds, and microalgae.