US2009325296A1PendingUtilityA1

Electrospun electroactive polymers for regenerative medicine applications

63
Assignee: NEW JERSEY TECH INSTPriority: Mar 25, 2008Filed: Mar 25, 2009Published: Dec 31, 2009
Est. expiryMar 25, 2028(~1.7 yrs left)· nominal 20-yr term from priority
Inventors:Treena ArinzehNorbert WeberMichael Jaffe
A61L 27/16C12N 2506/1307C12N 2533/30C12N 2506/1353A61L 27/3834C12N 2501/13C12N 5/0068A61L 27/3895A61L 2400/12A61L 27/383C12N 5/0619
63
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Claims

Abstract

Due to the size and complexity of tissues such as the spinal cord and articular cartilage, specialized constructs incorporating cells as well as smart materials may be a promising strategy for achieving functional recovery. Aspects of the present invention describe the use of an electroactive, or piezoelectric, material that will act as a scaffold for stem cell induced tissue repair. Embodiments of the inventive material can also act alone as an electroactive scaffold for repairing tissues. The piezoelectric material of the present invention acts as a highly sensitive mechanoelectrical transducer that will generate charges in response to minute vibrational forces.

Claims

exact text as granted — not AI-modified
1 . An electroactive structure for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material
 wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and   wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.   
   
   
       2 . The electroactive structure according to  claim 1 , wherein the biocompatible synthetic piezoelectric polymeric material is poly(vinylidene fluoride trifluoroethylene) copolymer. 
   
   
       3 . The electroactive structure according to  claim 1 , wherein the matrix fibers is a non-woven mesh of nanofibers. 
   
   
       4 . The electroactive structure according to  claim 1 , wherein the three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material is formed by electrospinning. 
   
   
       5 . The electroactive structure according to  claim 1 , wherein the isolated differentiable cells are multipotent human mesenchymal cells. 
   
   
       6 . The electroactive structure according to  claim 5 , wherein the human mesenchymal stem cells are isolated from human bone marrow. 
   
   
       7 . The electroactive structure according to  claim 5 , wherein the isolated differentiable human mesenchymal cells have a CD44+ CD34− CD45− phenotype. 
   
   
       8 . The electroactive structure according to  claim 5 , wherein the mature cell phenotype comprises a chondrogenic cell phenotype. 
   
   
       9 . The electroactive structure according to  claim 5 , wherein the chonodrogenic cell phenotype on the structure produces at least one glycosaminoglycan. 
   
   
       10 . The electroactive structure according to  claim 1 , wherein the mature cell phenotype comprises a neuronal cell phenotype. 
   
   
       11 . A composition for use in tissue engineering, comprising
 (a) Isolated differentiable cells, and   (b) a supporting electroactive scaffold for growing the isolated differentiable cells, the supporting scaffold comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material   wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and   wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.   
   
   
       12 . The composition according to  claim 11 , wherein the biocompatible synthetic piezoelectric polymeric material is poly(vinylidene fluoride trifluoroethylene) copolymer. 
   
   
       13 . The composition according to  claim 11 , wherein the three dimensional matrix of fibers is a non-woven mesh of nanofibers. 
   
   
       14 . The composition according to  claim 11 , wherein the three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material is formed by electrospinning. 
   
   
       15 . The composition according to  claim 11 , wherein the isolated differentiable cells are multipotent human mesenchymal cells. 
   
   
       16 . The composition according to  claim 15 , wherein the human mesenchymal stem cells are isolated from human bone marrow. 
   
   
       17 . The composition according to  claim 15 , wherein the isolated differentiable human mesenchymal cells have a CD44+ CD34− CD45− phenotype. 
   
   
       18 . The composition according to  claim 15 , wherein the mature cell phenotype comprises a chondrogenic cell phenotype. 
   
   
       19 . The composition according to  claim 15 , wherein the chonodrogenic cell phenotype on the structure produces at least one glycosaminoglycan. 
   
   
       20 . The composition according to  claim 11 , wherein the mature cell phenotype comprises a neuronal cell phenotype. 
   
   
       21 . A method of making an implantable electroactive scaffold, the method comprising the steps:
 (a) isolating differentiable human cells from a human donor;   (b) preparing a three-dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material to form a cell scaffold;   (c) seeding the cell scaffold with the isolated differentiable human cells; and   (d) growing the differentiable human cells on the cell scaffold so that the differentiable human cells differentiate into a mature cell phenotype on the scaffold.   
   
   
       22 . The method according to  claim 21 , wherein the differentiable human cells in step (a) are multipotent human mesenchymal cells. 
   
   
       23 . The method according to  claim 22 , wherein step (a) further comprises the step of obtaining the differentiable human mesenchymal cells from bone marrow. 
   
   
       24 . The method according to  claim 22 , wherein the differentiable human mesenchymal cells in step (a) have a CD44+ CD34− CD45− phenotype. 
   
   
       25 . The method according to  claim 21 , wherein the biocompatible synthetic piezoelectric polymeric material in step (b) is poly(vinylidene fluoride trifluoroethylene) copolymer. 
   
   
       26 . The method according to  claim 21 , wherein the three dimensional matrix of fibers in step (b) is a non-woven mesh of nanofibers. 
   
   
       27 . The method according to  claim 21 , wherein the three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material is formed by electrospinning. 
   
   
       28 . The method according to  claim 21 , wherein the mature cell phenotype in step (d) comprises a chondrogenic cell phenotype. 
   
   
       29 . The method according to  claim 28 , wherein the chonodrogenic cell phenotype in step (d) produces at least one glycosaminoglycan. 
   
   
       30 . The method according to  claim 21 , wherein the mature cell phenotype in step (d) comprises a neuronal cell phenotype.

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