Synthetic Dog/Human Chimeric C-C Chemokine Receptor 2B

Abstract

Disclosed herein are polynucleotides encoding a chimeric C—C chemokine receptor 2B, the encoded polypeptides thereof and methods of making and using said polynucleotides and polypeptides.

1 - 7 . (canceled) 
     
     
         8 . An isolated chimeric CCR2B polypeptide encoded by a nucleotide sequence selected from the group consisting of:
 (a) a nucleotide sequence encoding an amino acid sequence having at least 86% identity with the amino acid sequence set forth in SEQ ID NO:2; and   (b) a nucleotide sequence that hybridizes with the complement of the nucleotide sequence of (a) under the following conditions: 6×SSC at 45° C. and washed at least once with 0.2×SSC, 0.1% SDS at 50° C.   
     
     
         9 . The isolated chimeric CCR2B polypeptide of  claim 8 , wherein the nucleotide sequence of (a) encodes the amino acid sequence set forth in SEQ ID NO:2. 
     
     
         10 . The isolated chimeric CCR2B polypeptide of  claim 8 , wherein said polypeptide is capable of mediating MCP-1-dependent calcium mobilization. 
     
     
         11 . An isolated polypeptide fragment comprising at least 15 contiguous amino acids from SEQ ID NO:2, wherein the fragment is selected from the group of consisting of:
 (a) a fragment containing at least a portion of the sequence of amino acids 1-35 of SEQ ID NO:2 contiguous with at least a portion of the sequence of amino acids 36-300 of SEQ ID NO:2;   (b) a fragment containing at least a portion of the sequence of amino acids 36-300 of SEQ ID NO:2 contiguous with at least a portion of the sequence of amino acids 301-360 of SEQ ID NO:2; and   (c) a fragment containing at least a portion of the sequence of amino acids 1-35 of SEQ ID NO:2 contiguous with the entire sequence of amino acids 36-300 of SEQ ID NO:2 contiguous with at least a portion of the sequence of amino acids 301-360 of SEQ ID NO:2.   
     
     
         12 . (canceled)