Standard material for a particle analyzer

Abstract

A standard material that is used for judging an abnormal portion in a particle analyzer is described. The standard material comprises first standard particles to be fluorescence-stained by a fluorescence-staining treatment and second standard particles that have preliminarily contained a fluorescence dye. A method and an analyzer that can judge an abnormal portion in a particle analyzer by using such a standard material are also described.

1 . A standard material for a particle analyzer, the particle analyzer carrying out a fluorescence-staining treatment on measuring particles contained in a biological sample and analyzing the fluorescence-stained measuring particles, comprising: first standard particles which can be fluorescence-stained by the fluorescence-staining treatment; second standard particles which have preliminarily contained a fluorescence dye; and a buffer solution. 
   
   
       2 . The standard material according to  claim 1 , wherein the second standard particles actually are not stained by the fluorescence-staining treatment. 
   
   
       3 . The standard material according to  claim 1 , wherein the measuring particles comprise the first measuring particles and the second measuring particles, the fluorescence-stained first standard particles are similar in a fluorescence intensity to the fluorescence-stained first measuring particles, and the second standard particles are similar in a fluorescence intensity to the fluorescence-stained second measuring particles. 
   
   
       4 . The standard material according to  claim 3 , wherein the first standard particles are similar in a scattered light intensity to the first measuring particles, and the second standard particles are similar in a scattered light intensity to the second measuring particles. 
   
   
       5 . The standard material according to  claim 3 , wherein the biological sample is urine, the first measuring particles are selected from the group consisting of white blood cells, red blood cells, epithelial cells and columnar cells, and the second measuring particles are bacteria. 
   
   
       6 . The standard material according to  claim 1 , wherein the first standard particles are at least one kind of particles selected from the group consisting of vinyl acetate polymer particles, polyacrylamide particles, hydrophilic vinyl polymer particles, latex particles and silica particles, and the second standard particles are fluorescent latex particles. 
   
   
       7 . The standard material according to  claim 1 , wherein the particle analyzer comprises: a measuring sample preparation unit for preparing a measuring sample by mixing the biological sample with a dye; a light source for irradiating the measuring sample with light; and a fluorescence detector for detecting fluorescence from the measuring sample. 
   
   
       8 . The standard material according to  claim 7 , wherein the particle analyzer comprises a scattered light detector for detecting scattered light from the measuring sample. 
   
   
       9 . The standard material according to  claim 1 , wherein the buffer solution includes a dispersibility-improving agent. 
   
   
       10 . The standard material according to  claim 9 , wherein the dispersibility-improving agent is a surfactant.