US2009313723A1PendingUtilityA1

Methods for altering organ mass, controlling fertility and enhancing asexual reproduction in plants

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Assignee: UNIV CALIFORNIAPriority: Jan 8, 1999Filed: Apr 12, 2007Published: Dec 17, 2009
Est. expiryJan 8, 2019(expired)· nominal 20-yr term from priority
Inventors:Robert L. FischerYukiko Mizukami
C07K 14/415C12N 15/8229C12N 15/8222C12N 15/8261Y02A40/146C12N 15/829C12N 15/827C12N 15/8289
62
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Claims

Abstract

The invention provides methods of altering organ mass, controlling fertility and enhancing asexual reproduction in plants through the modulation of plant growth and cell proliferation. The methods involve producing transgenic plants comprising a recombinant expression cassette containing an ANT nucleic acid linked to a plant promoter.

Claims

exact text as granted — not AI-modified
1 - 20 . (canceled) 
     
     
         21 . A method of increasing cell proliferation in a plant, the method comprising introducing into the plant an expression cassette comprising a heterologous plant promoter operably linked to a heterologous AINTEGUMENTA (ANT) nucleic acid encoding an ANT polypeptide, wherein the polypeptide is encoded by a polynucleotide identical to a polynucleotide that is amplified using an ANT nucleic acid as a template by PCR using a first primer comprising SEQ ID NO:6 or SEQ ID NO:7 and a second primer comprising SEQ ID NO:8; and
 selecting a plant with increased cell number.   
     
     
         22 . The method of  claim 21 , wherein the increased cell proliferation results in increased plant size, mass or fertility; and the method comprises the step of selecting plants with increased size, mass or fertility. 
     
     
         23 . The method of  claim 21 , wherein the heterologous plant promoter is heterologous to the plant. 
     
     
         24 . The method of  claim 21 , wherein the heterologous plant promoter is heterologous to the ANT nucleic acid. 
     
     
         25 . The method of  claim 21 , wherein the heterologous plant promoter is constitutive. 
     
     
         26 . The method of  claim 21 , wherein the heterologous plant promoter is inducible. 
     
     
         27 . The method of  claim 21 , wherein the heterologous plant promoter is a tissue-specific promoter. 
     
     
         28 . The method of  claim 27 , wherein the tissue-specific promoter is a root-specific promoter. 
     
     
         29 . The method of  claim 27 , wherein the tissue-specific promoter is a vegetative organ-specific promoter. 
     
     
         30 . The method of  claim 27 , wherein the tissue-specific promoter is a floral organ-specific promoter. 
     
     
         31 . The method of  claim 27 , wherein the tissue-specific promoter is a seed-specific promoter. 
     
     
         32 . The method of  claim 27 , wherein the tissue-specific promoter is an ovule-specific promoter. 
     
     
         33 . The method of  claim 21 , wherein the heterologous ANT nucleic acid encodes an ANT polypeptide having at least 80% sequence identity to SEQ ID NO:2 or SEQ ID NO:5. 
     
     
         34 . The method of  claim 21 , wherein the template is selected from  Arabidopsis  genomic DNA and  Arabidopsis  cDNA. 
     
     
         35 . A plant comprising a heterologous tissue-specific plant promoter operably linked to a heterologous AINTEGUMENTA (ANT) nucleic acid encoding an ANT polypeptide, wherein the polypeptide is encoded by a polynucleotide identical to a polynucleotide that is amplified using an ANT nucleic acid as a template by PCR using a first primer comprising SEQ ID NO:6 or SEQ ID NO:7 and a second primer comprising SEQ ID NO:8. 
     
     
         36 . The plant of  claim 35 , wherein the heterologous plant promoter is heterologous to the plant. 
     
     
         37 . The plant of  claim 35 , wherein the heterologous plant promoter is heterologous to the ANT nucleic acid. 
     
     
         38 . The plant of  claim 35 , wherein the heterologous plant promoter is constitutive. 
     
     
         39 . The plant of  claim 35 , wherein the heterologous plant promoter is inducible. 
     
     
         40 . The plant of  claim 35 , wherein the tissue-specific promoter is a root-specific promoter. 
     
     
         41 . The plant of  claim 35 , wherein the tissue-specific promoter is a vegetative organ-specific promoter. 
     
     
         42 . The plant of  claim 35 , wherein the tissue-specific promoter is a floral organ-specific promoter. 
     
     
         43 . The plant of  claim 35 , wherein the tissue-specific promoter is a seed-specific promoter. 
     
     
         44 . The plant of  claim 35 , wherein the tissue-specific promoter is an ovule-specific promoter. 
     
     
         45 . The plant of  claim 35 , wherein the heterologous ANT nucleic acid encodes an ANT polypeptide having at least 80% sequence identity to SEQ ID NO:2 or SEQ ID NO:5. 
     
     
         46 . A recombinant expression cassette comprising a heterologous tissue-specific plant promoter operably linked to a heterologous AINTEGUMENTA (ANT) nucleic acid encoding an ANT polypeptide, wherein the polypeptide is encoded by a polynucleotide identical to a polynucleotide that is amplified using an ANT nucleic acid as a template by PCR using a first primer comprising SEQ ID NO:6 or SEQ ID NO:7 and a second primer comprising SEQ ID NO:8, wherein the recombinant expression cassette promotes the tissue-specific expression of the ANT polypeptide in a plant. 
     
     
         47 . The recombinant expression cassette of  claim 46 , wherein the heterologous plant promoter is heterologous to the plant. 
     
     
         48 . The recombinant expression cassette of  claim 46 , wherein the heterologous plant promoter is heterologous to the ANT nucleic acid. 
     
     
         49 . The recombinant expression cassette of  claim 46 , wherein the heterologous plant promoter is constitutive. 
     
     
         50 . The recombinant expression cassette of  claim 46 , wherein the heterologous plant promoter is inducible. 
     
     
         51 . The recombinant expression cassette of  claim 46 , wherein the tissue-specific promoter is a root-specific promoter. 
     
     
         52 . The recombinant expression cassette of  claim 46 , wherein the tissue-specific promoter is a vegetative organ-specific promoter. 
     
     
         53 . The recombinant expression cassette of  claim 46 , wherein the tissue-specific promoter is a floral organ-specific promoter. 
     
     
         54 . The recombinant expression cassette of  claim 46 , wherein the tissue-specific promoter is a seed-specific promoter. 
     
     
         55 . The recombinant expression cassette of  claim 46 , wherein the tissue-specific promoter is an ovule-specific promoter. 
     
     
         56 . The recombinant expression cassette of  claim 46 , wherein the heterologous ANT nucleic acid encodes an ANT polypeptide having at least 80% sequence identity to SEQ ID NO:2 or SEQ ID NO:5.

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