Heart20049410 polypeptides and methods of making the same

Abstract

Novel full-length cDNAs are provided. cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information useful for analyzing the functions of the polypeptide.

1 . A substantially pure polypeptide comprising the amino acid sequence of SEQ ID NO:2 or a partial peptide thereof wherein the signal peptide has been removed. 
     
     
         2 . A polypeptide produced by the expression of a nucleic acid in a cell, wherein the nucleic acid comprises a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or a partial peptide thereof wherein the signal sequence has been removed. 
     
     
         3 . A method for producing a substantially pure polypeptide comprising the amino acid sequence of SEQ ID NO:2 or a partial peptide thereof wherein the signal peptide has been removed, said method comprising:
 i) transforming a cell with a polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or a partial peptide thereof wherein the signal sequence has been removed;   ii) culturing the transformed cell; and   iii) recovering an expression product.   
     
     
         4 . The method of  claim 3 , wherein the transformed polynucleotide is a vector comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or a partial peptide thereof wherein the signal sequence has been removed.