Peptides for Detection of Antibody to Porcine Reproductive Respiratory Syndrome Virus

Abstract

The invention provides compositions and methods for the detection and quantification of PRRSV antibodies and antibody fragments using polypeptides.

1 . A method of detecting a porcine reproductive and respiratory syndrome virus (PRRSV) polypeptide or PRRSV in a sample comprising:
 (a) contacting one or more antibodies that specifically bind to at least one epitope of an PRRSV polypeptide, wherein said polypeptide consists of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:18, or SEQ ID NO:13 with the sample under conditions that allow polypeptide/antibody complexes to form;   (b) detecting polypeptide/antibody complexes;   wherein the detection of polypeptide/antibody complexes is an indication that PRSSV or a PRSSV polypeptide is present in the sample.   
     
     
         2 . The method of  claim 1 , wherein the one or more antibodies are monoclonal antibodies, polyclonal antibodies, or antibody fragments. 
     
     
         3 . The method of  claim 1 , wherein the sample is a biological sample, serum, whole blood, milk, meat juice, sputum, lung lavage fluid, lung tissue, tonsil tissue, or lymph node tissue. 
     
     
         4 . The method of  claim 1 , further comprising contacting the complexes of (a) with an indicator reagent comprising prior to the performance of (b). 
     
     
         5 . The method of  claim 4 , wherein the indicator reagent that generates a measurable signal. 
     
     
         6 . The method of  claim 1 , wherein the amount of PRRSV polypeptide or PRRSV in the test sample is determined. 
     
     
         7 . The method of  claim 1 , wherein the one or more antibodies are attached to a substrate. 
     
     
         8 . The method of  claim 1 , wherein the method comprises an assay selected from the group of assays consisting of a reversible flow chromatographic binding assay, an enzyme linked immunosorbent assay, a radioimmunoassay, a hemagglutination assay, a western blot assay, a fluorescence polarization immunoassay, and an indirect immunofluorescence assay.