Novel centrerosome-associated protein and applications thereof

Abstract

A method for diagnosing a genetic disease associated with disturbances in mitotic spindle organization or with cell division anomalies or both, which comprises demonstrating a functional alteration of the gene encoding an ASAP protein comprising at least the following steps of: obtaining DNA containing the gene encoding the ASAP protein from a biological sample; bringing said DNA into contact with a probe, and under conditions for hybridization between the DNA and the probe; and detecting the hybrid formed; and wherein the ASAP protein is selected from the group consisting of a human protein having sequence SEQ ID NO:1 and proteins having a sequence exhibiting at least 80% identity or at least 90% similarity with entire SEQ ID NO. 1.

1 - 39 . (canceled) 
     
     
         40 . A method for diagnosing a genetic disease associated with disturbances in mitotic spindle organization or with cell division anomalies or both, which comprises demonstrating a functional alteration of the gene encoding an ASAP protein comprising at least the following steps of:
 a) obtaining DNA containing the gene encoding the ASAP protein from a biological sample;   b) bringing said DNA into contact with a probe, and under conditions for hybridization between the DNA and the probe; and   c) detecting the hybrid formed; and wherein the ASAP protein is selected from the group consisting of a human protein having sequence SEQ ID NO: 1 and proteins having a sequence exhibiting at least 80% identity or at least 90% similarity with entire SEQ ID NO. 1.   
     
     
         41 . The method of claim  28 , wherein step b) comprises an amplification step carried out using a pair of primers, and step c) comprises is a step of detecting the amplified nucleic acids formed. 
     
     
         42 . The method of claim  28 , further comprising a step of isolating and sequencing the gene encoding the ASAP protein from the sample. 
     
     
         43 . The method of claim  29 , wherein the pair of primers is selected from the group consisting of the sequences SEQ ID NOS: 31 to 43. 
     
     
         44 . The method of claim  28 , wherein the sequence of the protein has a sequence exhibiting at least 90% identity or at least 95% similarity with entire SEQ ID No. 1.