Polypeptides homologous to vegf and bmp1

Abstract

The present invention involves the identification and preparation of vascular endothelial growth factor-E (VEGF-E). VEGF-E is a novel polypeptide related to vascular endothelial growth factor (VEGF) and bone morphogenetic protein 1. VEGF-E has homology to VEGF including conservation of the amino acids required for activity of VEGF. VEGF-E can be useful in wound repair, as well as in the generation and regeneration of tissue.

1 . An isolated polypeptide comprising amino acids 52-65 of SEQ ID NO. 2. 
     
     
         2 . An isolated polypeptide comprising amino acids 118-125 of SEQ ID NO. 2. 
     
     
         3 . An isolated polypeptide comprising amino acids 260-273 of SEQ ID NO. 2. 
     
     
         4 . An isolated polypeptide comprising an amino acid sequence encoded by a nucleic acid molecule that hybridizes under stringent conditions to SEQ ID NO. 3 or SEQ ID NO. 4, wherein the polypeptide promotes proliferation of fibroblasts or survival of endothelial cells. 
     
     
         5 . The isolated polypeptide of  claim 1 ,  2 ,  3 , or  4 , further comprising a carrier. 
     
     
         6 . The isolated polypeptide of  claim 5 , wherein the carrier is a pharmaceutically acceptable carrier. 
     
     
         7 . An isolated nucleic acid molecule comprising a nucleotide sequence encoding amino acids 52-65 of SEQ ID NO. 2. 
     
     
         8 . An isolated nucleic acid molecule comprising a nucleotide sequence encoding amino acids 118-125 of SEQ ID NO. 2. 
     
     
         9 . An isolated nucleic acid molecule comprising a nucleotide sequence encoding amino acids 260-273 of SEQ ID NO. 2. 
     
     
         10 . An isolated nucleic acid molecule comprising a nucleotide sequence that hybridizes under stringent conditions to SEQ ID NO. 3 or SEQ ID NO. 4, wherein the nucleic acid molecule encodes a polypeptide that promotes proliferation of fibroblasts or survival of endothelial cells. 
     
     
         11 . A vector comprising the isolated nucleic acid of  claim 7 ,  8 ,  9 , or  10 . 
     
     
         12 . A host cell comprising the vector of  claim 11 .