US2009263881A1PendingUtilityA1
Alpha-Amylase Mutants
Est. expiryOct 30, 2017(expired)· nominal 20-yr term from priority
Inventors:Torben Vedel BorchertAllan SvendsenCarsten AndersenBjarne Ronfeldt NielsenTorben Lauesgaard NissenSoren Kjaerulff
C12Y 302/01001C12N 9/2417C11D 3/38636C07K 14/32C11D 3/386C11D 3/38609
61
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Claims
Abstract
The invention relates to a variant of a parent Termamyl-like α-amylase, which exhibits an alteration in at least one of the following properties relative to said parent α-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca 2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
Claims
exact text as granted — not AI-modified1 - 39 . (canceled)
40 . A variant alpha-amylase, which variant has alpha-amylase activity, has at least 90% homology with the amino acid sequence shown in SEQ ID. NO. 3, and which comprises one or more of the following substitutions (using SEQ SD NO:3 for numbering):
(a) R179A, D, N, C, E, Q, G, H, I, L, K, M, F, P,
S, T, W, Y, or V;
(b) G180A, D, R, N, C, E, Q, H, I, L, K, M, F, P,
S, T, W, Y, or V;
(c) I181A, R, N, C, E, Q, G, H, L, K, M, F, P, S,
T, W, Y, or V;
(d) G182A, R, D, N, C, E, Q, H, I, L, K, M, F, P,
S, T, W, Y, or V.
41 . A variant alpha-amylase, which variant has alpha-amylase activity, has at least 95% homology with the amino acid sequence shown in SEQ ID. NO. 3, and which comprises one or more of the following substitutions (using SEQ ID NO:3 for numbering):
(a) R179A, D, N, C, E, Q, G, H, I, L, K, M, F, P,
S, T, W, Y, or v;
(b) G180A, D, R, N, C, E, Q, H, I, L, K, M, F, P,
S, T, W, Y, or V;
(c) I181A, R, N, C, E, Q, G, H, L, K, M, F, P, S,
T, W, Y, or V;
(d) G182A, R, D, N, C, E, Q, H, I, L, K, M, F, P,
S, T, W, Y, or V.
42 . The variant of claim 40 , wherein the variant comprises a substitution of R179A,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y, or V.
43 . The variant of claim 40 , wherein the variant comprises a substitution of G180A,D,R,N,C,E,Q,H,I,L,K,M,F,P,S,T,W,Y, or V.
44 . The variant of claim 40 , wherein the variant comprises a substitution of I181A,R,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y, or V.
45 . The variant of claim 40 , wherein the variant comprises a substitution of G182A,R,D,N,C,E,Q,H,I,L,K,M,F,P,S,T,W,Y, or V.
46 . The variant of claim 41 , wherein the variant comprises a substitution of R179A,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,TlW,Y, or V.
47 . The variant of claim 41 , wherein the variant comprises a substitution of G180A,D,R,N,C,E,Q,H,I,L,K,M,F,P,S,T,W,Y, or V.
48 . The variant of claim 41 , wherein the variant comprises a substitution of I181A,R,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,or V.
49 . The variant of claim 41 , wherein the variant comprises a substitution of G182A,R,D,N,C,E,Q,H,I,L,K,M,F,P,S,T,W,Y, or V.Join the waitlist — get patent alerts
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