US2009253615A1PendingUtilityA1

No-modified hemoglobins and uses therefor

Assignee: UNIV DUKEPriority: Sep 15, 1995Filed: Apr 21, 2009Published: Oct 8, 2009
Est. expirySep 15, 2015(expired)· nominal 20-yr term from priority
Y10T436/105831A61K 38/00A61K 38/44A61K 31/04C07K 14/00A61K 31/195C07K 14/805G01N 33/721A61K 47/6445A61K 35/18A61K 31/00A61K 47/6901A61K 31/095G01N 21/631G01N 21/76
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Claims

Abstract

Nitrosylhemoglobin can be produced by introducing gaseous NO into an aqueous solution of hemoglobin. It has been demonstrated that nitrosylhemoglobin in aqueous solution can be converted to SNO-hemoglobin upon introduction of oxygen to the solution, as is postulated to occur in the lungs. Nitrosylhemoglobin can be used in methods to produce the physiological effects of NO, for example, to reduce vasoconstriction and to inhibit platelet aggregation.

Claims

exact text as granted — not AI-modified
1 . A method for increasing delivery of oxygen and decreasing delivery of NO in a mammal, comprising administering to the mammal a composition comprising a low molecular weight thiol and isolated hemoglobin, wherein the thiol is cysteine or N-acetylcysteine. 
   
   
       2 . A method for increasing delivery of oxygen and increasing delivery of NO in a mammal, the method comprising administering to the mammal a composition comprising a low molecular weight nitrosothiol and isolated hemoglobin, wherein the low molecular weight nitrosothiol is S-nitrosocysteine, S-nitrosohomocysteine, or S-nitrosocysteinylglycine.

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