US2009253592A1PendingUtilityA1

Method and apparatus for treating a biological sample with a liquid reagent

59
Individually held — no corporate assignee on recordPriority: Dec 23, 2003Filed: Jun 12, 2009Published: Oct 8, 2009
Est. expiryDec 23, 2023(expired)· nominal 20-yr term from priority
Inventors:Brian Howard Kram
B01L 2200/0684B01L 3/508B01L 2300/0822B01L 2200/0642G01N 1/312G01N 1/2813G01N 2035/00158
59
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Claims

Abstract

A method and apparatus for thin film fluid processing of biological samples without rinsing between treatments is provided. An apparatus having a treatment zone for treating a biological sample with a liquid reagent, comprising first and second substrates having facing surfaces defining a space therebetween in which the biological sample may be treated with the liquid reagent, wherein the first substrate comprises a relatively fluid impermeable element while the second substrate comprises a relatively flexible gas permeable element.

Claims

exact text as granted — not AI-modified
1 . An apparatus for treating a biological sample with a liquid reagent, comprising first and second substrates having facing surfaces defining a space therebetween in which said biological sample may be treated with said liquid reagent, wherein said first substrate comprises a relatively rigid fluid impermeable element for supporting said biological sample while said second substrate comprises a relatively flexible gas permeable element backed by a liquid impermeable element. 
   
   
       2 . The apparatus of  claim 1 , wherein said first substrate comprises a glass slide. 
   
   
       3 . The apparatus of  claim 2 , wherein said biological specimen comprises a sectioned tissue sample carried on said slide. 
   
   
       4 . The apparatus of  claim 2 , wherein said biological sample comprises a cytological preparation carried on said slide. 
   
   
       5 . The apparatus of  claim 2 , wherein said biological sample comprises a tissue sample array carried on said slide. 
   
   
       6 . The apparatus of  claim 2 , wherein said biological sample comprises a DNA microarray carried on said slide. 
   
   
       7 . The apparatus of  claim 2 , wherein said biological sample comprises a protein microarray carried on said slide. 
   
   
       8 . The apparatus of  claim 1 , wherein said second substrate comprises a gas permeable hydrophobic element. 
   
   
       9 . The apparatus of  claim 1 , wherein said second substrate comprises a gas permeable hydrophobic porous flexible membrane. 
   
   
       10 . The apparatus of  claim 9 , wherein said gas permeable hydrophobic porous flexible membrane comprises a polytetrafluoroethylene tape or sheet. 
   
   
       11 . The apparatus of  claim 9 , wherein said gas permeable hydrophobic porous flexible membrane comprises a micro-porous semipermeable tape or sheet. 
   
   
       12 . The apparatus of  claim 9 , wherein said gas permeable hydrophobic porous flexible membrane is laminated to a liquid impermeable backing element. 
   
   
       13 . The apparatus according to  claim 1 , wherein said liquid reagent comprises a biological stain or biological reagent. 
   
   
       14 . The apparatus according to  claim 1 , wherein said liquid reagent comprises an aqueous based solution. 
   
   
       15 . The apparatus according to  claim 1 , wherein said liquid reagent is spread substantially uniformly between said facing surfaces. 
   
   
       16 . The apparatus according to  claim 1 , wherein said liquid reagent is substantially free of gas bubbles. 
   
   
       17 . The apparatus according to  claim 1 , wherein said liquid reagent has a film thickness of about 7-360 μm. 
   
   
       18 . The apparatus of  claim 1 , wherein said liquid reagent has a volume of about 100-300 microliters. 
   
   
       19 . The apparatus of  claim 1 , wherein said liquid reagent has a volume of about 5-50 microliters. 
   
   
       20 . The apparatus of  claim 1 , wherein said liquid reagent has a volume of about 20 microliters.

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