Trichoderma Reesei Phytase Enzymes, Nucleic Acids Encoding Such Phytase Enzymes, Vectors and Host Cells Incorporating Same and Methods of Making and Using Same

Abstract

A novel DNA is provided which encodes an enzyme having phytase activity isolated from Trichoderma. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.

1 .- 32 . (canceled) 
     
     
         33 . An isolated and/or recombinant polynucleotide comprising a nucleotide sequence (i) having at least 85% identity to a nucleotide sequence as disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3), or (ii) being capable of hybridizing to a probe derived from the nucleotide sequence disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3) under conditions of high stringency, or (iii) being complementary to the nucleotide sequence disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         34 . The polynucleotide of  claim 33 , wherein said nucleotide sequence has at least 90% identity to the nucleotide sequence disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         35 . The polynucleotide of  claim 33 , wherein said polynucleotide encodes an enzyme having phytase activity, wherein the enzyme is derived from a  Trichoderma  source. 
     
     
         36 . The polynucleotide of  claim 35 , wherein the enzyme is derived from  Trichoderma reesei.    
     
     
         37 . An isolated and/or recombinant polynucleotide encoding an enzyme having phytase activity, wherein the enzyme is derived from a  Trichoderma  source, and wherein said polynucleotide has (i) at least 55% identity to a nucleotide sequence as disclosed in  FIG. 1  (SEQ ID NO: 1) or (ii) is capable of hybridizing to a probe derived from the nucleotide sequence disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3) under conditions of high stringency, or (iii) is complementary to the nucleotide sequence disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         38 . The polynucleotide of  claim 37 , wherein said polynucleotide has at least 75% identity to a nucleotide sequence as disclosed in  FIG. 1  (SEQ ID NO: 1). 
     
     
         39 . The polynucleotide of  claim 37 , wherein said polynucleotide has at least 85% identity to a nucleotide sequence as disclosed in  FIG. 1  (SEQ ID NO: 1). 
     
     
         40 . An expression construct including a polynucleotide sequence (i) having at least 55% identity to a nucleotide sequence as disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3), or (ii) being capable of hybridizing to a probe derived from the nucleotide sequence disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3) under conditions of medium to high stringency, or (iii) being complementary to the nucleotide sequence disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         41 . The expression construct of  claim 40 , wherein said polynucleotide has at least 75% identity to a nucleotide sequence as disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         42 . The expression construct of  claim 40 , wherein said polynucleotide has at least 85% identity to a nucleotide sequence as disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         43 . A vector including the expression construct of  claim 40 . 
     
     
         44 . A host cell transformed with the vector of  claim 43 . 
     
     
         45 . A probe for use in detecting nucleic acid sequences coding for an enzyme having phytase activity derived from a microbial source, comprising: a nucleotide sequence (i) having at least 55% identity to a nucleotide sequence as disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3), or (ii) being capable of hybridizing to a polynucleotide including a sequence as disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3) under conditions of medium to high stringency, or (iii) being complementary to the nucleotide sequence disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         46 . The probe of  claim 45 , wherein said nucleotide sequence has at least 75% identity to a nucleotide sequence as disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         47 . The probe of  claim 45 , wherein said nucleotide sequence has at least 85% identity to a nucleotide sequence as disclosed in  FIG. 1 ,  2  or  3  (SEQ ID NOS: 1, 2, or 3). 
     
     
         48 . The probe of  claim 45 , wherein said microbial source is a fungal source. 
     
     
         49 . The probe of  claim 48 , wherein said fungal source is a  Trichoderma  species.