Savinase variants having an improved wash performance on egg stains

Abstract

Subtilase variants having an improved wash performance on egg stains. These subtilases are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.

1 . A subtilase enzyme variant having the following modifications S3T+V4I+S99D+S101R+S103A+V104I+G160S+V205I+L217D or S3T+V4I+S99D+S101R+S103A+V104I+G160S+A194P+V205I+L217D (BASBPN numbering). 
     
     
         2 . The subtilase enzyme variant according to  claim 1 , wherein the parent subtilase is subtilisin 309 (BLSAVI). 
     
     
         3 . The subtilase enzyme variant according to  claim 1  which has the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4. 
     
     
         4 . The subtilase according to  claim 1 , further comprising at least one modification in one of the positions 27, 36, 56, 76, 87, 95, 96, 97, 98, 100, 102, 120, 123, 159, 167, 170, 206, 218, 222, 224, 232, 235, 236, 245, 248, 252 and 274 (BASBPN numbering). 
     
     
         5 . The subtilase according to  claim 4 , wherein the modification is selected from the group consisting of K27R, *36D, S56P, N76D, S87N, G97N, H120D, N123S, G159D, Y167, R170, Q206E, N218S, M222S, M222A, T224S, A232V, K235L, Q236H, Q245R, N248D, N252K and T274A (BASBPN numbering). 
     
     
         6 . An isolated polynucleotide comprising a polynucleotide encoding the subtilase defined in  claim 1 . 
     
     
         7 . An isolated polynucleotide encoding a subtilase, selected from the group consisting of
 the polynucleotide of SEQ ID NO:1 and the polynucleotide of SEQ ID NO:3.   
     
     
         8 . A nucleic acid construct comprising the nucleic acid sequence of any of  claims 6 - 7  operably linked to one or more control sequences capable of directing the expression of the subtilase in a suitable host. 
     
     
         9 . A recombinant expression vector comprising the nucleic acid construct of  claim 8 , a promoter, and transcripbonal and translational stop signals. 
     
     
         10 . A recombinant host cell comprising the nucleic acid construct of  claim 8  or the expression vector of  claim 9 . 
     
     
         11 . The host cell according to  claim 10 , which is a bacterium. 
     
     
         12 . The host cell according to  claim 10 , which is a fungus or yeast. 
     
     
         13 . A method for producing the subtilase according to  claim 1 , the method comprising:
 (a) cultivating a recombinant host cell as defined in  claim 10  under conditions conducive to the production of the subtilase; and
 (b) recovering the subtilase. 
   
     
     
         14 . A cleaning or detergent composition comprising the subtilase according to  claim 1 . 
     
     
         15 . The composition according to  claim 14 , which additionally comprises a cellulase, lipase, cutinase, oxidoreductase, another protease, an amylase, a pectate lyase, a mannanase or a mixture thereof. 
     
     
         16 .- 18 . (canceled) 
     
     
         19 . A method for cleaning or dish washing, washing a hard surface or laundry, the method comprising contacting the hard surface or the laundry with the composition defined in  claim 14 . 
     
     
         20 . A method for removal of egg stains from a hard surface or from laundry, the method comprising contacting the egg stain-containing hard surface or the egg stain-containing laundry with the composition defined in  claim 14 .