Stabilizing solutions for submicronic particles, methods for making the same and methods of stabilizing submicronic particles

Abstract

Stabilizing solutions for submicronic particles, methods for making the same and methods of stabilizing submicronic particles is disclosed.

1 .- 7 . (canceled) 
     
     
         8 . A method of making a sub micronic particle stabilizing solution, which comprises the steps of
 (a) obtaining fresh biological tissue;   (b) macerating the biological tissue in water to form a suspension containing the extracts of the biological tissue;   (c) removing from the suspension suspended particles greater than one micron to obtain a clear concentrated extract;   (d) diluting the concentrated extract with deionized water in a ratio ranging from the original to 1 to 10 dilution;   (e) adjusting the temperature to 25 degrees Celsius;   (f) adjusting the pH of the diluted extract to between 5.5 to 7.5 pH; and   (g) measuring the open circuit potential to ensure that that potential lies within the range of +0.1 to +0.2 volt; and   (h) measuring the total organic carbon content to ensure that the content is at least 18,000 parts per million in solution.   
     
     
         9 . A method of making a sub micronic particle stabilizing solution as claimed in  claim 8 , in which the biological tissue is macerated by at least one method from a group of macerating methods which consists of methods, grinding, blending, milling, microwave treatment, ultrasonication, sonication, pounding, pressure extrusion, freezing-thawing, irradiation, heat treatment, osmolysis, enzymatic lysis, chemical lysis, vacuum lysis, and differential pressure lysis. 
     
     
         10 . A method of making a sub micronic particle stabilizing solution in which the removal of suspended particles is achieved by filtering the suspension through a sub micronic filter element. 
     
     
         11 . A method of stabilizing sub micronic particles which comprises the steps of
 (a) dispersing sub micronic particles in deionized water to form a dispersion in which the concentration of the particles is in the range of 150 to 60000 ppm;   (b) adding the dispersion to the stabilizing solution of claim  1  to obtain a resultant in which the concentration of the particles ranges from 5 to 300 ppm;   (c) mixing the resultant for a period of 30 minutes to three hours to obtain a suspension of stabilized solid sub micronic particles.   
     
     
         12 . A method of stabilizing sub micronic metal particles, during their synthesis, which comprises the steps of
 dispersing salt of the metal in deionized water to form a solution;   adding the formed solution to the stabilizing solution in accordance with claim  1 , to obtain a resultant in which the concentration of the metal is in the range from 5 to 300 ppm and the effective dilution of the stabilizing solution is in the range of 1:1 to 1:10;   adding a reducing agent to the resultant; and   mixing the resultant for a period of 30 minutes to three hours to obtain a suspension of stabilized solid submicronic particles.   
     
     
         13 . A method as claimed in  claim 11 , where the sub micronic particles are particles selected from a group of particles selected from transition metals, alkali metals, alkaline earth metals, rare earth metals, metalloids, a combination of metals, metallic compounds. 
     
     
         14 . A method as claimed in  claim 11 , where the sub micronic particles and the stabilizing solution is added during the synthesis of the nano particles by a process from a group of process which includes a chemical process, a physical process and a biological process. 
     
     
         15 . A method as claimed in  claim 11 , where the sub micronic particles are silver ions and the step includes dispersing a silver salt in deionized water having conductivity of less than 3 micro siemens. 
     
     
         16 . A method as claimed in  claim 11 , where the sub micronic particles are gold ions and the step includes dispersing a gold salt in deionized water. 
     
     
         17 . A method as claimed in  claim 11 , in which, the aqueous extract is treated with a non polar solvent, typically n-cyclohexane. 
     
     
         18 . A method as claimed in  claim 12 , in which, the reducing agent is at least one reducing agent selected from a group containing the stabilizing solution, citric acid, borohydride, sodium sulfide sodium acetate.