US2009209008A1PendingUtilityA1

Thermus thermophilus nucleic acid polymerases

Assignee: APPLIED BIOSYSTEMSPriority: Nov 30, 2001Filed: Aug 18, 2008Published: Aug 20, 2009
Est. expiryNov 30, 2021(expired)· nominal 20-yr term from priority
C12P 19/34C12N 9/1252C12Y 207/07007C12Q 1/686Y10S435/975
64
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Claims

Abstract

The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of Thermus thermophilus , and nucleic acids encoding those polymerases, as well as methods for using the polymerases and nucleic acids.

Claims

exact text as granted — not AI-modified
1 - 55 . (canceled) 
     
     
         1 . A method for thermocyclic amplification of nucleic acid comprising:
 (a) contacting a nucleic acid with a thermostable polypeptide having any one of SEQ ID NO: 13-24 under conditions suitable for amplification of said nucleic acid; and   (b) amplifying the nucleic acid.   
     
     
         2 . The method of  claim 1  wherein the thermocyclic amplification of the nucleic acid includes cycles of denaturation, primer annealing and primer extension. 
     
     
         3 . The method of  claim 1  wherein the thermocyclic amplification of the nucleic acid is performed by Strand Displacement Amplification. 
     
     
         4 . The method of  claim 1  wherein thermocyclic amplification of the nucleic acid is performed by Polymerase Chain Reaction.

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