US2009203615A1PendingUtilityA1

Use of activity dependent neurotrophic factor for enhancing learning and memory: pre-and post-natal administration

Assignee: US GOV HEALTH & HUMAN SERVPriority: May 31, 2000Filed: Aug 25, 2008Published: Aug 13, 2009
Est. expiryMay 31, 2020(expired)· nominal 20-yr term from priority
A61P 43/00A61P 9/00A61P 31/18A61P 9/10A61P 3/10A61P 35/00A61P 25/00A61P 25/14A61P 25/16A61P 25/28A61P 25/32A61P 25/18A61P 25/08A61P 21/04A61K 38/00A61P 17/02C07K 14/475
57
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Claims

Abstract

The present invention provides methods for improving performance (e.g., learning and/or memory) using ADNF polypeptides, by treating the subject prenatally or postnatally with an Activity Dependent Neurotrophic Factor (ADNF) polypeptide in an amount sufficient to improve postnatal learning and/or memory of the subject.

Claims

exact text as granted — not AI-modified
1 . A method for improving learning and/or memory in a subject, the method comprising the step of administering postnatally to the subject an Activity Dependent Neurotrophic Factor (ADNF) polypeptide in an amount sufficient to improve postnatal learning and/or memory of the subject, wherein the ADNF polypeptide is a member selected from the group consisting of:
 (a) an ADNF I polypeptide comprising an active core site having the following amino acid sequence:   
       
         
           
                 
                 
               
                   Ser-Ala-Leu-Leu-Arg-Ser-Ile-Pro-Ala; 
                   (SEQ ID NO: 1) 
                 
             
                
               
            
           
         
         (b) an ADNF III polypeptide comprising an active core site having the following amino acid sequence: 
       
       
         
           
                 
                 
               
                   Asn-Ala-Pro-Val-Ser-Ile-Pro-Gln. 
                   (SEQ ID NO:2) 
                 
             
                
               
            
           
         
         . 
       
     
     
         2 . (canceled) 
     
     
         3 . The method of  claim 1 , wherein the ADNF polypeptide is an ADNF I polypeptide. 
     
     
         4 . The method of  claim 3 , wherein the active core site of the ADNF I polypeptide comprises at least one D-amino acid. 
     
     
         5 . The method of  claim 3 , wherein the active core site of the ADNF I polypeptide comprises all D-amino acids. 
     
     
         6 . The method of  claim 3 , wherein the ADNF I polypeptide is Ser-Ala-Leu-Leu-Arg-Ser-Ile-Pro-Ala (SEQ ID NO:1). 
     
     
         7 . The method of  claim 3 , wherein the ADNF I polypeptide is selected from the group consisting of: 
       
         
           
                 
                 
               
                   (SEQ ID NO: 14) 
                     
                 
                 
                 
               
                   Val-Leu-Gly-Gly-Gly-Ser-Ala-Leu-Leu-Arg-Ser- 
                     
                 
                   Ile-Pro-Ala; 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO: 15) 
                     
                 
                 
                 
               
                   Val-Glu-Glu-Gly-Ile-Val-Leu-Gly-Gly-Gly-Ser-Ala- 
                     
                 
                   Leu-Leu-Arg-Ser-Ile-Pro-Ala; 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO: 16) 
                     
                 
                 
                 
               
                   Leu-Gly-Gly-Gly-Ser-Ala-Leu-Leu-Arg-Ser-Ile-Pro- 
                     
                 
                   Ala; 
                 
                     
                 
                 
               
                   (SEQ ID NO: 17) 
                     
                 
                 
                 
               
                   Gly-Gly-Gly-Ser-Ala-Leu-Leu-Arg-Ser-Ile-Pro-Ala; 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO: 18) 
                     
                 
                 
                 
               
                   Gly-Gly-Ser-Ala-Leu-Leu-Arg-Ser-Ile-Pro-Ala; 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO: 19) 
                     
                 
                 
                 
               
                   Gly-Ser-Ala-Leu-Leu-Arg-Ser-Ile-Pro-Ala; 
                     
                 
                   and 
                     
                 
                 
                 
               
                   (SEQ ID NO: 1) 
                     
                 
                 
                 
               
                   Ser-Ala-Leu-Leu-Arg-Ser-Ile-Pro-Ala. 
                     
                 
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
               
            
           
         
       
     
     
         8 . The method of  claim 3 , wherein the ADNF I polypeptide comprises up to about 20 amino acids at least one of the N-terminus and the C-terminus of the active core site. 
     
     
         9 . The method of  claim 1 , wherein the ADNF polypeptide is an ADNF III polypeptide. 
     
     
         10 . The method of  claim 9 , wherein the ADNF III polypeptide is Asn-Ala-Pro-Val-Ser-Ile-Pro-Gln (SEQ ID NO:2). 
     
     
         11 . The method of  claim 9 , wherein the active core site of the ADNF III polypeptide comprises at least one D-amino acid. 
     
     
         12 . The method of  claim 9 , wherein the active core site of the ADNF III polypeptide comprises all D-amino acids. 
     
     
         13 . The method of  claim 9 , wherein the ADNF III polypeptide is a member selected from the group consisting of: 
       
         
           
                 
                 
               
                   (SEQ ID NO: 20) 
                     
                 
                 
                 
               
                   Gly-Gly-Asn-Ala-Pro-Val-Ser-Ile-Pro-Gln; 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO: 21) 
                     
                 
                 
                 
               
                   Leu-Gly-Gly-Asn-Ala-Pro-Val-Ser-Ile-Pro-Gln- 
                     
                 
                   Gln-Ser; 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO: 22) 
                     
                 
                 
                 
               
                   Leu-Gly-Leu-Gly-Gly-Asn-Ala-Pro-Val-Ser-Ile-Pro- 
                     
                 
                   Gln-Gln-Ser; 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO: 23) 
                     
                 
                 
                 
               
                   Ser-Val-Arg-Leu-Gly-Leu-Gly-Gly-Asn-Ala-Pro-Val- 
                     
                 
                   Ser-Ile-Pro-Gln-Gln-Ser; 
                 
                   and 
                 
                 
                 
               
                   (SEQ ID NO: 2) 
                     
                 
                 
                 
               
                   Asn-Ala-Pro-Val-Ser-Ile-Pro-Gln. 
                     
                 
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
               
            
           
         
       
     
     
         14 . The method of  claim 9 , wherein the ADNF III polypeptide comprises up to about 20 amino acids at least one of the N-terminus and the C-terminus of the active core site. 
     
     
         15 - 21 . (canceled) 
     
     
         22 . The method of  claim 1 , wherein the subject is afflicted with a neuropathology. 
     
     
         23 . The method of  claim 1 , wherein the subject has Alzheimer's disease. 
     
     
         24 . The method of  claim 1 , wherein the subject has Down's syndrome. 
     
     
         25 . The method of  claim 1 , wherein the subject is normal. 
     
     
         26 . The method of  claim 25 , wherein the subject is old. 
     
     
         27 . The method of  claim 1 , wherein the method improves short term memory. 
     
     
         28 . The method of  claim 1 , wherein the method improves reference memory. 
     
     
         29 . The method of  claim 1 , wherein the ADNF polypeptide is administered intranasally or orally. 
     
     
         30 - 59 . (canceled)

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