Method and device for selectively targeting cells within a three-dimensional specimen

Abstract

The invention provides an apparatus for electromagnetically affecting a particle of interest in a specimen. The apparatus includes (a) a stage capable of supporting the specimen; (b) a detector including at least one camera, wherein the detector is capable of resolving a particle of interest within the specimen; (c) a means for locating the particle of interest in three dimensions; (d) a means for focusing electromagnetic radiation to a focal volume within the specimen; and (e) a means for adjusting the relative positions of the stage and electromagnetic radiation focusing means, thereby positioning the particle of interest within the focal volume.

1 . An apparatus for electromagnetically affecting a particle of interest in a specimen, comprising:
 (a) a stage capable of supporting the specimen;   (b) a detector comprising at least one camera, wherein the detector is capable of resolving a particle of interest within the specimen;   (c) a means for locating the particle of interest in three dimensions;   (d) a means for focusing electromagnetic radiation to a focal volume within the specimen; and   (e) a means for adjusting the relative positions of the stage and electromagnetic radiation focusing means, thereby positioning the particle of interest within the focal volume.