Insect resistant coton plants and methods of detecting the same

Abstract

The present application relates to an insect resistant transgenic cotton event designated COT202. The application also relates to polynucleotides which are characteristic of the COT202 event, plants comprising said polynucleotides, and methods of detecting the COT202 event.

1 . A method of detecting plant material derived from the COT202 event comprising:
 (a) obtaining a sample for analysis;   (b) providing DNA from the sample;   (c) providing a first primer and a second designed to bind to a polynucleotide comprising the sequence of SEQ ID NO: 1 or SEQ ID NO: 2 when said polynucleotide is single stranded;   (d) amplifying the region which lies between the sites at which the primers bind; and   (e) detecting the presence of the amplification product;   
       whereby the presence of the amplification product is indicative that the sample is derived from the COT202 event. 
     
     
         2 . The method according to  claim 1  wherein the first primer has the sequence of SEQ ID NO: 3 and the second primer has the sequence of SEQ ID NO: 4. 
     
     
         3 . A method of detecting plant material derived from the COT202 event comprising:
 (a) obtaining a sample for analysis;   (b) providing a probe designed to bind to the complement of a polynucleotide comprising the sequence of SEQ ID NO: 1 or SEQ ID NO: 2 when said polynucleotide is single stranded;   (c) hybridising said probe with the sample; and   (d) detecting whether the probe has hybridised;   
       whereby the hybridisation of the probe is indicative that the sample is derived from the COT202 event. 
     
     
         4 . The method according to  claim 3  wherein the sequence of the probe is selected from the group comprising SEQ ID NO: 7 and SEQ ID NO: 8. 
     
     
         5 . The method according to  claim 3  wherein the probe hybridises to the sample under stringent hybridisation conditions. 
     
     
         6 . A kit of parts comprising a means for detecting the presence in a sample of plant material derived from event COT202. 
     
     
         7 . The kit of parts according to  claim 6  comprising a means for detecting the presence in a sample of a polynucleotide comprising the sequence of SEQ ID NO: 1 or SEQ ID NO: 2 or a protein encoded by said polynucleotide.