System, Method and Apparatus for Purifying Biological Fluids Such as Blood and Constituents Thereof

Abstract

Provided herein is an innovative hemoperfusion system and method including a thermal-fused broad-spectrum biocidal iodinated interactive polymer for the treatment of biological contaminants in body fluids. In one exemplary embodiment, the system and method utilizes a Triosyn® thermal fused broad-spectrum iodinated interactive polymer, included in a hemoperfusion column, for devitalizing high levels of cell-free microorganisms in whole blood and biological fluids in relation with the characterization of blood cells viability and function post-treatment

1 . A method for decontaminating blood, comprising:
 a) providing a hemoperfusion unit containing a low-density chamber including an effective amount of demand disinfectant, said demand disinfectant being an iodinated resin;   b) separating a unit of blood infected with a quantity of bacteria or viral particles to provide a red blood cell concentrate and a plasma concentrate;   c) discarding the plasma concentrate;   d) diluting the red blood cell concentrate with an aqueous solution;   e) filtering said diluted red blood cell concentrate through said hemoperfusion unit at a selected flow rate,   
       wherein the amount of bacteria or viral particles is reduced by greater than 90%. 
     
     
         2 . The method of  claim 1 , wherein the amount of bacteria or viral particles is reduced by greater than 95%. 
     
     
         3 . The method of  claim 1 , wherein the amount of bacteria or viral particles is reduced by greater than 99%. 
     
     
         4 . The method of  claim 1 , wherein the bacteria or viral particles are selected from the group consisting of  Staphylococcus aureus, E. coli  and MS2. 
     
     
         5 . The method of  claim 1  further comprising gravity filtering red blood cell concentrate through a leukocyte filter to remove leukocytes prior to diluting the red blood cell concentrate. 
     
     
         6 . The method of  claim 1  further comprising gravity filtering red blood cell concentrate through a leukocyte filter to remove leukocytes after diluting the red blood cell concentrate. 
     
     
         7 . The method of  claim 1 , wherein the aqueous solution is a saline solution. 
     
     
         8 . The method of  claim 1 , wherein the said flow rate is 50 ml/mm. 
     
     
         9 . The method of  claim 1 , wherein the said flow rate is 20 ml/mm. 
     
     
         10 . The method of  claim 1 , wherein the a low-density chamber further contains hydrodynamic diffusers. 
     
     
         11 . A method for decontaminating blood, comprising:
 a) providing a hemoperfusion unit containing a low-density chamber including an effective amount of demand disinfectant, said demand disinfectant being an iodinated resin;   b) sonicating a unit of blood infected with a quantity of bacteria or viral particles:   c) separating the unit of blood to provide a red blood cell concentrate and a plasma concentrate;   d) discarding the plasma concentrate;   e) diluting the red blood, cell concentrate with an aqueous solution;   f) filtering said diluted red blood cell concentrate through said hemoperfusion unit at a selected flow rate. wherein the amount of bacteria or viral particles is reduced by greater than 90%.   
     
     
         12 . A method of disinfecting red blood cells, comprising passing a unit of red blood cells infected with a quantity of bacteria or viral particles through a hemoperfusion unit containing a demand disinfectant iodinated resin, wherein the amount of bacteria or viral particles is reduced by greater than 90%. 
     
     
         13 . The method of  claim 12 , wherein the hemoperfusion unit contains hydrodynamic diffusers that maximize contact time between said bacterial or viral particles and said demand disinfectant iodinated resin. 
     
     
         14 . The method as in  claim 1  wherein said unit of whole blood is provided by a donor; said method further comprising: transfusing said red blood concentrate of step e) into said donor or a donee. 
     
     
         15 . A filtration method, comprising: providing a contaminated product having high concentrations of microorganisms; filtering said contaminated product with a hemoperfusion unit to remove said microorganisms; said hemoperfusion unit comprising: a low-density column including an effective amount of demand disinfectant; said disinfecting being an iodinated resin, wherein the concentration of microorganisms is reduced by greater than 90%. 
     
     
         16 . A portable hemoperfusion filtration system, comprising:
 a) a plasma separator for removing plasma from whole blood to provide a red blood cell concentrate;   b) a dilution device for diluting said red blood cell concentrate;   c) a chamber containing a demand disinfectant, said demand disinfectant being an iodinated resin contained in said column; and   d) a pump for moving said diluted red blood cell concentrate a blood constitute through said chamber column at a selected flow rate.   
     
     
         17 . The filtration system of  claim 1 , wherein the chamber further contains hydrodynamic diffusers. 
     
     
         18 . A hemoperfusion filtration chamber comprising a demand disinfectant iodinated resin and a hydrodynamic diffuser, wherein the diffuser functions to increase the contact efficacy between the iodinated resin and bacteria in blood that is passed through said chamber.