siRNA targeting tumor necrosis factor receptor superfamily member 1A

Abstract

Efficient sequence specific gene silencing of siRNA targeting tumor necrosis factor receptor superfamily member 1A is possible through the use of siRNA technology. By selecting particular siRNAs by rationale design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes.

1 . An siRNA molecule, wherein said siRNA molecule consists of: (a) a duplex region; and (b) 0-4 overhang regions that each contain six or fewer nucleotides, wherein the duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region together form said duplex region and said duplex region is 19-30 base pairs in length and said antisense region comprises a sequence that is the complement of SEQ ID NO: 441. 
     
     
         2 . The siRNA molecule of  claim 1 , wherein said antisense region and said sense region are each 19-25 bases in length. 
     
     
         3 . The siRNA molecule of  claim 1 , wherein said antisense region and said sense region are each 19 bases in length. 
     
     
         4 . The siRNA molecule of  claim 1 , wherein said siRNA molecule has at least one overhang region. 
     
     
         5 . The siRNA molecule of  claim 1 , wherein said siRNA molecule has no overhang regions. 
     
     
         6 . The siRNA molecule of  claim 2 , wherein said siRNA molecule has at least one overhang region. 
     
     
         7 . The siRNA molecule of  claim 2 , wherein said siRNA molecule has no overhang regions. 
     
     
         8 . The siRNA molecule of  claim 3 , wherein said siRNA molecule has at least one overhang region. 
     
     
         9 . The siRNA molecule of  claim 3 , wherein said siRNA molecule has no overhang regions. 
     
     
         10 . A chemically synthesized siRNA molecule that directs cleavage of mRNA via RNA interference, wherein: (a) each strand of said siRNA molecule is between 19 and 30 nucleotides in length; and (b) one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 441. 
     
     
         11 . The chemically synthesized siRNA molecule of  claim 10 , wherein each strand of said siRNA molecule is 19 nucleotides in length. 
     
     
         12 . An siRNA molecule, wherein said siRNA molecule consists of: (a) a sense region, wherein said sense region consists of a sequence that is SEQ ID NO: 441, and an antisense region, wherein said antisense region consists of a sequence that is the complement of SEQ ID NO: 441; and (b) from zero to four overhang regions, wherein each overhang region is six or fewer nucleotides in length. 
     
     
         13 . The siRNA molecule of  claim 12 , wherein said siRNA molecule has no overhang regions. 
     
     
         14 . The siRNA molecule of  claim 12 , wherein said siRNA molecule has one overhang region. 
     
     
         15 . The siRNA molecule of  claim 12 , wherein said siRNA molecule has two overhang regions. 
     
     
         16 . A pool of at least two siRNAs, wherein said pool comprises a first siRNA molecule and a second siRNA molecule, wherein said first siRNA molecule is the siRNA molecule of  claim 1  and said second siRNA molecule consists of a second duplex region and 0-4 overhang regions that each contain six or fewer nucleotides, wherein the second duplex region of said second siRNA molecule comprises a second sense region and a second antisense region, wherein said second sense region and said second antisense region together form said second duplex region and said second duplex region is 19-30 base pairs in length and said second antisense region comprises a sequence that is the complement of a sequence selected from the group consisting of SEQ ID NO: 438-440 and 442-447. 
     
     
         17 . The pool of  claim 16 , wherein said first siRNA molecule and said second siRNA molecule each have no overhang regions. 
     
     
         18 . The pool of  claim 16 , wherein said duplex region of said first siRNA molecule and said second duplex region are each 19-25 base pairs in length. 
     
     
         19 . The pool of  claim 16 , wherein said duplex region of said first siRNA molecule and said second duplex region are each 19 base pairs in length. 
     
     
         20 . The pool of  claim 16 , wherein the second antisense region comprises a sequence that is the complement to SEQ ID NO: 443.